Robinson I M, Fernandez J M
Department of Physiology and Biophysics, Mayo Clinic, Rochester, Minnesota 55905.
Curr Opin Neurobiol. 1994 Jun;4(3):330-6. doi: 10.1016/0959-4388(94)90093-0.
The development of micro-voltammetry to detect the release of secretory products from single cells has yielded surprising information, which suggests that the release of secretory products is regulated after the fusion of secretory vesicles with the plasma membrane. This technique has also been used to demonstrate that the release of secretory products can occur during transient fusion events, which leads one to question the current models for membrane recycling. In the past year, strong evidence has emerged in support of a role for rab3 and G alpha i3 proteins in regulating a putative scaffold of proteins that cause bilayer fusion during exocytosis. These findings parallel the biochemical identification of several new cytosolic, secretory vesicle and plasma membrane proteins that may also play a role in regulating fusion.
用于检测单细胞分泌产物释放的微伏安法的发展产生了惊人的信息，这表明分泌产物的释放在分泌囊泡与质膜融合后受到调节。该技术还被用于证明分泌产物的释放在瞬时融合事件期间可以发生，这使人对当前的膜回收模型产生质疑。在过去的一年里，出现了强有力的证据支持rab3和Gαi3蛋白在调节胞吐作用期间引起双层融合的假定蛋白质支架中的作用。这些发现与几种新的胞质、分泌囊泡和质膜蛋白的生化鉴定结果相似，这些蛋白也可能在调节融合中发挥作用。
