Kumakura S, Ishikura H, Tsumura H, Hayashi H, Endo J, Tsunematsu T
Third Division of Internal Medicine, Shimane Medical University, Izumo, Japan.
Leuk Lymphoma. 1994 Jun;14(1-2):171-80. doi: 10.3109/10428199409049665.
We examined c-myc protein expression in cell cycle phases during differentiation induction of HL-60 cells by flow cytometry using an indirect immunofluorescence method. In exponentially proliferating HL-60 cells, c-myc protein was expressed in a cell cycle dependent manner. During the differentiation induction of HL-60 cells with dimethylsulfoxide (DMSO), c-myc protein was rapidly down-regulated in the G1/0 specific phase prior to the appearance of differentiation associated markers. Our results indicate that c-myc protein functions in the G1/0 specific phase in cellular differentiation, and the rapid down-regulation of c-myc protein in G1/0 phase is closely associated with initial differentiation programs.
