Morita T, Kanagawa A, Fujii M, Ueki H
Department of Biochemistry, Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, Japan.
Biol Pharm Bull. 1994 May;17(5):724-6. doi: 10.1248/bpb.17.724.
Lipoprotein lipase (LPL), which is responsible for the hydrolysis of lipoprotein triacylglyceride, has been examined in Ehrlich ascites tumor cells. Low molecular weight dextran sulfate (DXS, M.W. 3.2 kDa) stimulates the release of the enzyme activity from the tumor cells into the incubation medium in a time-dependent manner. The stimulatory effect of DXS was markedly decreased by incubation with protein tyrosine kinase (TK) inhibitors, such as ST 638, biochanin A and amiloride. The activity of the partially purified TK preparation from the tumor cells was found to be increased following incubation with DXS in a manner which was both time- and dose-dependent. These results suggest that the stimulatory release of LPL activity by DXS is associated with the activation of TK in the tumor cells.
脂蛋白脂肪酶（LPL）负责脂蛋白三酰甘油的水解，已在艾氏腹水肿瘤细胞中进行了研究。低分子量硫酸葡聚糖（DXS，分子量3.2 kDa）以时间依赖性方式刺激该酶活性从肿瘤细胞释放到孵育培养基中。与蛋白酪氨酸激酶（TK）抑制剂如ST 638、鹰嘴豆芽素A和阿米洛利一起孵育后，DXS的刺激作用显著降低。发现与DXS孵育后，从肿瘤细胞中部分纯化的TK制剂的活性以时间和剂量依赖性方式增加。这些结果表明，DXS对LPL活性的刺激释放与肿瘤细胞中TK的激活有关。
