Mise K, Miyahara M
Eisei Shikenjo Hokoku. 1993(111):1-12.
Restriction endonucleases have been widely employed in almost all fields of genetic engineering including DNA mapping, cloning, sequencing, hybridization, amplification and diagnosis. The general characteristics of restriction endonucleases and their reactions are reviewed in this paper, together with their distribution in pathogenic bacteria. Many restriction endonucleases with novel specificity have been found in these bacteria in our laboratory. Some of them are commercially available and have been employed for the molecular biologist. Rapid method for detection of restriction endonucleases in pathogenic bacteria is also described.
限制性内切酶已广泛应用于基因工程的几乎所有领域，包括DNA图谱绘制、克隆、测序、杂交、扩增和诊断。本文综述了限制性内切酶的一般特性及其反应，以及它们在病原菌中的分布。我们实验室在这些细菌中发现了许多具有新特异性的限制性内切酶。其中一些已商业化并被分子生物学家所使用。本文还描述了病原菌中限制性内切酶的快速检测方法。
