Craig J M, Bickmore W A
MRC Human Genetics Unit, Western General Hospital, Edinburgh, Scotland.
Nat Genet. 1994 Jul;7(3):376-82. doi: 10.1038/ng0794-376.
Using fluorescent in situ suppression hybridization to metaphase chromosomes, we have directly shown that CpG islands are predominantly found in the early replicating (R band) regions of the genome. Conversely, late replicating (G band) DNA is sparsely populated with islands. The very highest concentration of CpG islands is in a subset of R bands, most of which are known as T bands. We suggest that there is an interdependence between the differences in island density and the behaviour of chromosomal domains. Our findings indicate which regions of the genome will yield the highest density of coding sequence information. An awareness of local island density may influence the choice of method for identifying exons in genomic DNA.
利用荧光原位抑制杂交技术对中期染色体进行研究，我们直接证明了CpG岛主要存在于基因组早期复制（R带）区域。相反，晚期复制（G带）DNA中的岛分布稀疏。CpG岛的最高浓度存在于R带的一个子集中，其中大部分被称为T带。我们认为岛密度差异与染色体结构域的行为之间存在相互依存关系。我们的研究结果表明基因组的哪些区域将产生最高密度的编码序列信息。了解局部岛密度可能会影响在基因组DNA中识别外显子的方法选择。
