Saito M, Hypolite J A, Wein A J, Levin R M
Division of Urology, University of Pennsylvania, School of Medicine, Philadelphia.
Neurourol Urodyn. 1994;13(3):297-305. doi: 10.1002/1520-6777(1994)13:3<297::aid-nau1930130311>3.0.co;2-y.
Partial outlet obstruction induces significant alteration in detrusor contractility. A mild obstruction can result in increased contractile force, whereas severe obstruction results in marked contractile dysfunction. The cellular mechanisms mediating these alterations in the contractile response are presently not known. In the current study, we have investigated the effect of both mild and severe partial outlet obstruction on detrusor contractility and correlated the contractile response to field stimulation, bethanechol, adenosine triphosphate (ATP), and KCl with the level of intracellular free calcium using FURA-2 fluorescence. Our results are as follows. Severe obstruction induced a significantly greater increase in bladder weight than mild obstruction. In general, mild outlet obstruction induced an increase in the contractile responses to field stimulation, bethanechol, ATP, and KCl, whereas severe outlet obstruction induced a decrease to field stimulation and ATP. In general, the stimulated increase in intracellular free calcium paralleled the contractile response. The results indicate that the alterations in the contractile response to field stimulation and pharmacological stimulation induced by partial outlet obstruction may be mediated by altered calcium translocation and intracellular release.
部分出口梗阻会导致逼尿肌收缩力发生显著改变。轻度梗阻可导致收缩力增加，而重度梗阻则会导致明显的收缩功能障碍。目前尚不清楚介导这些收缩反应改变的细胞机制。在本研究中，我们研究了轻度和重度部分出口梗阻对逼尿肌收缩力的影响，并使用FURA-2荧光将收缩反应与电场刺激、氨甲酰甲胆碱、三磷酸腺苷（ATP）和氯化钾刺激下的细胞内游离钙水平进行关联。我们的结果如下。重度梗阻比轻度梗阻导致膀胱重量增加更为显著。一般来说，轻度出口梗阻会使对电场刺激、氨甲酰甲胆碱、ATP和氯化钾的收缩反应增加，而重度出口梗阻则会使对电场刺激和ATP的反应降低。一般来说，刺激引起的细胞内游离钙增加与收缩反应平行。结果表明，部分出口梗阻引起的对电场刺激和药理刺激的收缩反应改变可能是由钙转运和细胞内释放改变介导的。
