Janny L, Vye P, Pouly J L, Hazout A, Dumont M, Nicollet B, Menezo Y
Unité de FIV, Polyclinique Hôtel Dieu, Clermont-Ferrand.
Contracept Fertil Sex. 1993 May;21(5):391-4.
Results of french multiple collaborative studies are reported to evaluate the possible benefits of coculture in human IVF program. Prospective randomized study of IVF with transfer on day two versus IVF with transfer on day 6 shows that coculture does not improve overall results but leads to a reduction of triple or quadruple pregnancies. When applied to patients with more than 4 failures of embryo transfers on day 2, delayed transfer of blastocyst results in a high rate of clinical pregnancy (39%). Transfers of frozen-thawed blastocysts result in 17.8% ongoing pregnancies. Coculture is also a powerful tool for understanding early embryo development: when fertilization arises from sperm with poor characteristics or from frozen sperm of donor, embryo development appears to be altered: in these situations, rate of normally developing embryo is reduced and early embryo development becomes independent of fertilization rate.
据报道，法国多项合作研究的结果旨在评估共培养在人类体外受精（IVF）程序中的潜在益处。对第二天移植的IVF与第六天移植的IVF进行的前瞻性随机研究表明，共培养并不能改善总体结果，但会导致三胎或四胎妊娠率降低。当应用于第二天胚胎移植失败超过4次的患者时，囊胚延迟移植会导致较高的临床妊娠率（39%）。冻融囊胚移植导致17.8%的持续妊娠。共培养也是理解早期胚胎发育的有力工具：当受精来自特征不佳的精子或供体冷冻精子时，胚胎发育似乎会受到影响：在这些情况下，正常发育的胚胎率会降低，早期胚胎发育变得与受精率无关。
