Cloning DNA binding proteins from cDNA expression libraries using oligonucleotide binding site probes.
作者信息
Cowell I G, Hurst H C
机构信息
Gene Transcription Laboratory, Hammersmith Hospital, Du Cane, London, UK.
出版信息
Methods Mol Biol. 1994;31:363-70. doi: 10.1385/0-89603-258-2:363.
PMID:7921033
Abstract
摘要
相似文献
1
Cloning DNA binding proteins from cDNA expression libraries using oligonucleotide binding site probes.
Methods Mol Biol. 1994;31:363-70. doi: 10.1385/0-89603-258-2:363.
2
Cloning sequence-specific DNA-binding factors from cDNA expression libraries using oligonucleotide binding site probes.
Methods Mol Biol. 1997;69:161-70. doi: 10.1385/0-89603-383-x:161.
3
Gene isolation by screening lambda gt11 expression libraries with DNA-binding site probes.
Methods Mol Biol. 1995;37:393-407. doi: 10.1385/0-89603-288-4:393.
4
Cloning genes for DNA binding proteins: dot-blotting to optimize cDNA expression library screening.克隆DNA结合蛋白的基因：斑点印迹法优化cDNA表达文库筛选
Anal Biochem. 1994 May 1;218(2):465-8. doi: 10.1006/abio.1994.1210.
5
Preparing lambda libraries for expression of proteins in prokaryotes or eukaryotes.制备用于在原核生物或真核生物中表达蛋白质的λ噬菌体文库。
Methods Mol Biol. 2003;221:271-87. doi: 10.1385/1-59259-359-3:271.
6
In vivo construction of cDNA libraries for use in the yeast two-hybrid system.用于酵母双杂交系统的cDNA文库的体内构建。
Yeast. 1999 Jun 15;15(8):715-20. doi: 10.1002/(SICI)1097-0061(19990615)15:8<715::AID-YEA406>3.0.CO;2-K.
7
An expression cloning method to identify monomeric GTP-binding proteins by GTP overlay.
Anal Biochem. 1997 Dec 15;254(2):187-91. doi: 10.1006/abio.1997.2422.
8
A procedure for second-round differential screening of cDNA libraries.一种用于cDNA文库第二轮差异筛选的方法。
Biotechniques. 1994 Feb;16(2):229-30, 232.
9
Production of cDNA libraries by electroporation.
Methods Mol Biol. 1995;47:67-79. doi: 10.1385/0-89603-310-4:67.
10
Preparation and ligand screening of a lambda gt11 lysogen library.λgt11溶原菌文库的制备及配体筛选
Methods Mol Biol. 1994;31:397-407. doi: 10.1385/0-89603-258-2:397.
Zotero 插件