Brennemann W, Sommer L, Stoffel-Wagner B, Bidlingmaier F, Klingmüller D
Institut für Klinische Biochemie, Universität Bonn, Germany.
Eur J Endocrinol. 1994 Sep;131(3):273-9. doi: 10.1530/eje.0.1310273.
Chronological changes in serum concentrations of inhibin, a gonadal glycoprotein hormone, were studied in healthy male volunteers (age 24-27 years). Secretion profiles of immunoreactive inhibin (ir-inhibin) were compared with those of luteinizing hormone (LH), follicle-stimulating hormone (FSH) and testosterone. Blood samples were collected every 15 min for 24 h. Serum inhibin concentrations were measured by a two-site immunoenzymatic assay with antibodies raised against distinct epitopes of the recombinant 1-32 amino acids of the alpha-subunit of human inhibin. The normal range for men was 0.79-3.1 U/l x 10(-3), the sensitivity of the assay was 0.1 U/l x 10(-3) (cv: within-assay, 6.8%; between-assay, 8.2%). Luteinizing hormone and FSH were measured by immunoradiometric assay and testosterone by radioimmunoassay. Secretion profiles of inhibin and testosterone were tested for diurnal variations by cosinor rhythmometry. Highest ir-inhibin concentrations were observed in the morning at 08.00 h, with peak values of 2.45-3.20 U/l x 10(-3). During the evening and the night, ir-inhibin levels were relatively low; lowest concentrations were observed between 01.00 h and 02.00 h at night: 1.20-1.86 U/l x 10(-3). Highest testosterone levels were observed in the morning (20.5-36.6 pmol/l), lowest concentrations were detected at night (7.35-12.6 pmol/l). Cosinor rhythmometry supported the suggestion that there is a clear circadian secretion of ir-inhibin and testosterone, respectively. The secretion pattern of ir-inhibin was analyzed by the Cluster pulse analysis computed algorithm, which identified four to seven inhibin pulses per day, depending on the person under observation.2+ volunteers follow a clear diurnal rhythm.(ABSTRACT TRUNCATED AT 250 WORDS)
在健康男性志愿者（年龄24 - 27岁）中研究了性腺糖蛋白激素抑制素血清浓度的时间变化。将免疫反应性抑制素（ir - 抑制素）的分泌情况与促黄体生成素（LH）、促卵泡激素（FSH）和睾酮的分泌情况进行比较。每15分钟采集一次血样，共采集24小时。采用双位点免疫酶测定法，用针对人抑制素α亚基重组1 - 32个氨基酸不同表位产生的抗体来测定血清抑制素浓度。男性的正常范围是0.79 - 3.1 U/l×10⁻³，该测定法的灵敏度为0.1 U/l×10⁻³（变异系数：批内，6.8%；批间，8.2%）。用免疫放射测定法测定促黄体生成素和促卵泡激素，用放射免疫测定法测定睾酮。通过余弦节律分析法检测抑制素和睾酮的分泌情况是否存在昼夜变化。上午8点观察到ir - 抑制素浓度最高，峰值为2.4 - 3.20 U/l×10⁻³。在傍晚和夜间，ir - 抑制素水平相对较低；夜间1点到2点之间观察到最低浓度：1.20 - 1.86 U/l×10⁻³。上午观察到睾酮水平最高（20.5 - 36.6 pmol/l），夜间检测到最低浓度（7.35 - 12.6 pmol/l）。余弦节律分析法支持了ir - 抑制素和睾酮分别有明显昼夜分泌的观点。用聚类脉冲分析计算算法分析ir - 抑制素的分泌模式，根据观察对象不同，每天可识别出4至7个抑制素脉冲。2名以上志愿者遵循明显的昼夜节律。（摘要截断于250字）
