Crawford B A, Handelsman D J
Department of Obstetrics and Gynaecology, University of Sydney, Australia.
Eur J Endocrinol. 1994 Oct;131(4):405-12. doi: 10.1530/eje.0.1310405.
In vitro studies indicate a physiological role for insulin-like growth factor I (IGF-I) in paracrine regulation of testicular function and recent clinical studies suggest a potential role for growth hormone (GH) and/or IGF-I in the treatment of hypogonadotrophic states in males. This study aimed to examine the effects of pretreatment with recombinant human GH (rhGH) or rhIGF-I on the response to gonadotrophins of the non-human primate testis in vivo. Using a balanced Latin square design with repeated measures, six prepubertal male hamadryas baboons (Papio hamadryas hamadryas) were treated in a cross-over sequence for periods of 18 days with daily im injections of rhGH (0.4 IU.kg-1.day-1), rhIGF-I (0.1 mg.kg-1.day-1) or saline with a 2-week washout period between each treatment. A single im injection of hCG (1500 IU) increased serum testosterone (p = 0.0002) but neither rhGH nor rhIGF-I influenced the timing or magnitude of this response (p > 0.5). A single im dose of FSH (75 IU) stimulated immunoreactive inhibin (p = 0.01) but also was unaffected in magnitude or timing by pretreatment with rhGH or rhIGF-I (p > 0.2). Circulating IGF-I levels were increased independently by hCG (p = 0.01) and FSH (p < 0.0001) administration. These findings indicate that neither GH nor IGF-I pre-treatment enhance acute gonadal responses to gonadotrophin stimulation of the prepubertal non-human primate testis in vivo. These findings suggest that GH or IGF-I treatment of hypogonadotrophic men without somatotrophin deficiency is unlikely to be beneficial.
体外研究表明胰岛素样生长因子I（IGF-I）在睾丸功能的旁分泌调节中具有生理作用，近期的临床研究提示生长激素（GH）和/或IGF-I在男性性腺功能减退状态的治疗中可能发挥作用。本研究旨在检测重组人生长激素（rhGH）或rhIGF-I预处理对非人灵长类动物睾丸在体内对促性腺激素反应的影响。采用平衡拉丁方设计并重复测量，6只青春期前雄性阿拉伯狒狒（Papio hamadryas hamadryas）按照交叉顺序接受为期18天的每日皮下注射rhGH（0.4 IU·kg⁻¹·d⁻¹）、rhIGF-I（0.1 mg·kg⁻¹·d⁻¹）或生理盐水治疗，每次治疗间隔2周的洗脱期。单次皮下注射人绒毛膜促性腺激素（hCG，1500 IU）可使血清睾酮升高（p = 0.0002），但rhGH和rhIGF-I均未影响该反应的时间或幅度（p > 0.5）。单次皮下注射促卵泡激素（FSH，75 IU）可刺激免疫反应性抑制素升高（p = 0.01），但rhGH或rhIGF-I预处理对其幅度或时间也无影响（p > 0.2）。hCG（p = 0.01）和FSH（p < 0.0001）给药均可独立升高循环IGF-I水平。这些发现表明，在体内，GH和IGF-I预处理均不能增强青春期前非人灵长类动物睾丸对促性腺激素刺激的急性性腺反应。这些发现提示，对无生长激素缺乏的性腺功能减退男性进行GH或IGF-I治疗不太可能有益。
