尿卟啉原III酶促形成过程中中间体的证据。

Evidence for an intermediate in the enzymatic formation of uroporphyrinogen III.

作者信息

Pichon C, Atshaves B P, Stolowich N J, Scott A I

机构信息

Department of Chemistry, Texas A&M University, College Station 77843-3255.

出版信息

Bioorg Med Chem. 1994 Mar;2(3):153-68. doi: 10.1016/s0968-0896(00)82011-8.

DOI:10.1016/s0968-0896(00)82011-8

PMID:7922129

Abstract

Evidence for an azafulvene intermediate in the enzymatic formation of Uroporphyrinogen III has been obtained. Using conditions to slow down the enzyme activity (high pH, low temperature), the transient species was trapped with ammonium ions as aminomethylbilane and with sodium borohydride as methylbilane, and observed by 13C-NMR.

摘要

已获得关于在尿卟啉原III酶促形成过程中氮杂富烯中间体的证据。利用减缓酶活性的条件（高pH值、低温），该瞬态物种分别用铵离子捕获为氨甲基联吡咯，用硼氢化钠捕获为甲基联吡咯，并通过13C核磁共振进行观测。