Barton D H, Bardin C O, Doller D
Department of Chemistry, Texas A & M University, College Station 77843-3255.
Bioorg Med Chem. 1994 Apr;2(4):259-66. doi: 10.1016/s0968-0896(00)82169-0.
In harmony with our studies on the activation of hydrocarbons by Gif chemistry, we have, in the first part of this paper, studied the mechanism of the lipoxygenase enzymes using soybean lipoxygenase as a model. We have shown with trimethyl phosphite that no free radical is released by the enzyme. In a second part, we have studied the mechanism of the alpha-ketoglutarate dependent enzymes and shown evidence for a mechanism involving the reduction of an intermediate hydroperoxide by the alpha-ketoglutaric acid.
与我们关于通过Gif化学活化碳氢化合物的研究一致，在本文的第一部分，我们以大豆脂氧合酶为模型研究了脂氧合酶的作用机制。我们用亚磷酸三甲酯表明该酶不会释放自由基。在第二部分中，我们研究了依赖α-酮戊二酸的酶的作用机制，并证明了一种涉及α-酮戊二酸还原中间氢过氧化物的机制。
