大鼠嗅梨状皮质中促性腺激素释放激素信使核糖核酸的存在

Presence of gonadotropin-releasing hormone mRNA in the rat olfactory piriform cortex.

作者信息

Choi W S, Kim M O, Lee B J, Kim J H, Sun W, Seong J Y, Kim K

机构信息

Department of Anatomy, College of Medicine, Gyeongsang National University, Chinju, South Korea.

出版信息

Brain Res. 1994 Jun 13;648(1):148-51. doi: 10.1016/0006-8993(94)91914-3.

DOI:10.1016/0006-8993(94)91914-3

PMID:7922516

Abstract

Gonadotropin releasing hormone (GnRH) neurons are known to be originated from the olfactory placode and to enter the forebrain regions during embryonic development. The present study aims to ascertain whether GnRH is locally expressed in the olfactory cortex. Northern blot hybridization and reverse transcription-polymerase chain reaction revealed that GnRH mRNA was present in the rat olfactory cortex as well as in the olfactory bulb. The predicted size of GnRH mRNA is similar to that detected in the hypothalamus. In situ hybridization histochemistry also showed that GnRH mRNA is highly concentrated in the olfactory piriform cortex. The present data indicate that GnRH is synthesized in the olfactory piriform cortex.

摘要

促性腺激素释放激素（GnRH）神经元已知起源于嗅基板，并在胚胎发育期间进入前脑区域。本研究旨在确定GnRH是否在嗅皮质中局部表达。Northern印迹杂交和逆转录聚合酶链反应显示，GnRH mRNA存在于大鼠嗅皮质以及嗅球中。GnRH mRNA的预测大小与在下丘脑中检测到的相似。原位杂交组织化学也表明，GnRH mRNA高度集中在嗅梨状皮质中。目前的数据表明，GnRH是在嗅梨状皮质中合成的。