Street M D, Donovan G R, Baldo B A, Sutherland S
Kolling Institute of Medical Research, Royal North Shore Hospital of Sydney, St Leonards, NSW, Australia.
Clin Exp Allergy. 1994 Jun;24(6):590-7. doi: 10.1111/j.1365-2222.1994.tb00957.x.
IgE antibody reactivities to the venoms of Myrmecia pilosula, Myrmecia nigrocincta, Myrmecia tarsata, Myrmecia pyriformis, Myrmecia simillima and Myrmecia gulosa have been identified in sera from subjects allergic to ant venom. Sera with IgE reactivity to only a single Myrmecia venom most often recognize M. pilosula venom although all six venoms appear capable of inducing IgE antibodies. The six different Myrmecia venoms were electrophoretically separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), Western blotted to nitrocellulose and probed with sera from ant venom-allergic subjects. Four to six IgE-binding components ranging in size from 2 to 25 kDa were identified in each of the venoms. Similarities in molecular weights of the IgE-binding components exist and close taxonomic relationships between the species suggest that common or similar peptides may be present in the different venoms.
在对蚁毒过敏的受试者血清中，已鉴定出针对多毛牛蚁、黑带牛蚁、跗节牛蚁、梨形牛蚁、似牛蚁和巨牛蚁毒液的IgE抗体反应性。对单一牛蚁毒液具有IgE反应性的血清最常识别多毛牛蚁毒液，尽管所有六种毒液似乎都能够诱导IgE抗体。通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）对六种不同的牛蚁毒液进行电泳分离，转移至硝酸纤维素膜上进行蛋白质印迹，并用蚁毒过敏受试者的血清进行检测。在每种毒液中鉴定出4至6种大小在2至25 kDa之间的IgE结合成分。IgE结合成分的分子量存在相似性，并且这些物种之间密切的分类关系表明不同毒液中可能存在共同或相似的肽。
