通过放射免疫扩散试验测定人血浆抗纤溶酶活性。
The measurement of human plasma antiplasmin activity by radial diffusion assay.
作者信息
Andes W A
出版信息
Thromb Haemost. 1978 Apr 30;39(2):437-49.
An assay of human antiplasmins has been developed utilizing radial diffusion of plasma from wells cut in plasmin-enriched, fibrinogen-agarose plates. After diffusion the fibrinogen is clotted. Zones of fibrin protected from background fibrinolysis develop as the result of plasma antiplasmin activity. A pooled plasma standard was taken to contain 100% antiplasmin activity. Antiplasmin activity of 52 normal subjects varied from 64 to 132%. Washed platelets contained 1-5% antiplasmin activity. Using antisera to precipitate individual inhibitors, physical methods of separation, and electrophoresis of plasma in agarose, several different proteins were found to have antiplasmin activity in this assay. Thus, alpha2-macroglobulin contributed 56%, alpha1-antitrypsin 20%, antithrombin III 2%, and other proteins 22% of the total antiplasmin activity. 1 ml of whole plasma neutralized 7.0 CTA units of plasmin.
利用从富含纤溶酶的纤维蛋白原 - 琼脂糖平板上所切孔中扩散血浆的方法，已开发出一种检测人抗纤溶酶的试验。扩散后，纤维蛋白原发生凝结。由于血浆抗纤溶酶活性，形成了免受背景纤维蛋白溶解影响的纤维蛋白区域。一个混合血浆标准品被设定为含有100%的抗纤溶酶活性。52名正常受试者的抗纤溶酶活性在64%至132%之间变化。洗涤后的血小板含有1 - 5%的抗纤溶酶活性。使用抗血清沉淀单个抑制剂、物理分离方法以及在琼脂糖中对血浆进行电泳，发现在该试验中有几种不同的蛋白质具有抗纤溶酶活性。因此，α2 - 巨球蛋白贡献了总抗纤溶酶活性的56%，α1 - 抗胰蛋白酶贡献了20%，抗凝血酶III贡献了2%，其他蛋白质贡献了22%。1毫升全血中和了7.0个酪蛋白溶解活性单位的纤溶酶。
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