Parsons L H, Justice J B
Department of Chemistry, Emory University, Atlanta, GA 30322.
Crit Rev Neurobiol. 1994;8(3):189-220.
The microdialysis technique has been widely used for the study of extracellular chemical events in various tissues. Until recently, however, little was known about the sampling characteristics of dialysis probes in vivo. The purpose of this review is to describe several theoretical models of in vivo microdialysis that consider both the diffusional resistances encountered in vivo and the effects of various active processes, such as metabolism, release and uptake, and transport across the blood brain barrier. The relevance of the models to practical in vivo microdialysis experiments is discussed. Several microdialysis techniques that quantitatively estimate extracellular analyte concentrations are also reviewed. These techniques are discussed in relation to the theoretical models, together with a discussion of the experimental factors that affect in vivo microdialysis experiments.
微透析技术已广泛应用于各种组织细胞外化学事件的研究。然而，直到最近，人们对体内透析探针的采样特性仍知之甚少。本综述的目的是描述几种体内微透析的理论模型，这些模型既考虑了体内遇到的扩散阻力，也考虑了各种活性过程的影响，如代谢、释放和摄取，以及跨血脑屏障的转运。讨论了这些模型与实际体内微透析实验的相关性。还综述了几种定量估计细胞外分析物浓度的微透析技术。结合理论模型对这些技术进行了讨论，并讨论了影响体内微透析实验的实验因素。
