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宫颈涂片中高致癌潜力和低致癌潜力人乳头瘤病毒DNA的半自动检测

Semiautomated detection of human papillomavirus DNA of high and low oncogenic potential in cervical smears.

作者信息

Terry G, Ho L, Szarewski A, Cuzick J

机构信息

Department of Molecular Pathology, University College London Medical School, UK.

出版信息

Clin Chem. 1994 Oct;40(10):1890-2.

PMID:7923767
Abstract

Detection of DNA from human papillomaviruses (HPV) of high and intermediate oncogenic risk in cervical smears may predict the presence of cervical cancer or may indicate precancerous changes. Here we describe a semiautomated polymerase chain reaction system for the detection and classification of HPV DNA that is present in clinically significant amounts in routine cervical scrapes.

摘要

在宫颈涂片检查中检测具有高致癌风险和中等致癌风险的人乳头瘤病毒(HPV)DNA,可能预示宫颈癌的存在,或提示癌前病变。在此,我们描述了一种半自动聚合酶链反应系统,用于检测和分类在常规宫颈刮片中存在具有临床意义数量的HPV DNA。

相似文献

1
Semiautomated detection of human papillomavirus DNA of high and low oncogenic potential in cervical smears.宫颈涂片中高致癌潜力和低致癌潜力人乳头瘤病毒DNA的半自动检测
Clin Chem. 1994 Oct;40(10):1890-2.
2
A new and sensitive method of screening for human papillomavirus infection.
Obstet Gynecol. 1989 Sep;74(3 Pt 1):410-4.
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DNA-EIA to detect high and low risk HPV genotypes in cervical lesions with E6/E7 primer mediated multiplex PCR.采用E6/E7引物介导的多重PCR技术,通过DNA酶免疫分析检测宫颈病变中的高危和低危HPV基因型。
J Clin Pathol. 1998 Jan;51(1):38-43. doi: 10.1136/jcp.51.1.38.
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Evaluation of human papillomavirus-consensus primers for HPV detection by the polymerase chain reaction.用于聚合酶链反应检测人乳头瘤病毒的人乳头瘤病毒通用引物的评估
Mol Cell Probes. 1999 Feb;13(1):9-21. doi: 10.1006/mcpr.1998.0203.
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Processing of long-stored archival cervical smears for human papillomavirus detection by the polymerase chain reaction.通过聚合酶链反应对长期保存的存档宫颈涂片进行人乳头瘤病毒检测的处理
Br J Cancer. 1995 Aug;72(2):412-7. doi: 10.1038/bjc.1995.347.
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A simplified and reliable HPV testing of archival Papanicolaou-stained cervical smears: application to cervical smears from cancer patients starting with cytologically normal smears.一种简化且可靠的对存档巴氏染色宫颈涂片进行人乳头瘤病毒检测的方法:应用于起始细胞学涂片正常的癌症患者的宫颈涂片。
Br J Cancer. 2000 Apr;82(8):1421-6. doi: 10.1054/bjoc.1999.1128.
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[Estimation of HPV infection presence in cytological smears in a group of asymptomatic women].[一组无症状女性细胞学涂片中人乳头瘤病毒(HPV)感染情况的评估]
Wiad Lek. 2001;54(9-10):508-15.
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HPV presence precedes abnormal cytology in women developing cervical cancer and signals false negative smears.在罹患宫颈癌的女性中,人乳头瘤病毒(HPV)的存在先于细胞学异常,并提示涂片检查假阴性。
Br J Cancer. 2001 Aug 3;85(3):398-404. doi: 10.1054/bjoc.2001.1926.
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Detection of high-risk subtypes of human papillomavirus in cervical swabs: routine use of the Digene Hybrid Capture assay and polymerase chain reaction analysis.宫颈拭子中人乳头瘤病毒高危亚型的检测:Digene杂交捕获检测法和聚合酶链反应分析的常规应用
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Human papillomavirus type 16 in cervical smears as predictor of high-grade cervical intraepithelial neoplasia [corrected].宫颈涂片中人乳头瘤病毒16型作为高级别宫颈上皮内瘤变的预测指标[已校正]
Lancet. 1992 Apr 18;339(8799):959-60. doi: 10.1016/0140-6736(92)91532-d.

引用本文的文献

1
High load for most high risk human papillomavirus genotypes is associated with prevalent cervical cancer precursors but only HPV16 load predicts the development of incident disease.大多数高危型人乳头瘤病毒基因型的高负荷与宫颈癌前病变的普遍存在相关,但只有HPV16负荷可预测新发疾病的发生。
Int J Cancer. 2007 Dec 15;121(12):2787-93. doi: 10.1002/ijc.23012.
2
HPV testing in primary screening of older women.老年女性初筛中的人乳头瘤病毒检测
Br J Cancer. 1999 Oct;81(3):554-8. doi: 10.1038/sj.bjc.6690730.
3
Nonisotopic detection and typing of human papillomavirus DNA in genital samples by the line blot assay. The Canadian Women's HIV study group.
通过线性印迹法对生殖器样本中的人乳头瘤病毒DNA进行非同位素检测和分型。加拿大女性艾滋病毒研究小组。
J Clin Microbiol. 1999 Jun;37(6):1852-7. doi: 10.1128/JCM.37.6.1852-1857.1999.