Satoh M, Langdon J J, Chou C H, McCauliffe D P, Treadwell E L, Ogasawara T, Hirakata M, Suwa A, Cohen P L, Eisenberg R A
Department of Medicine, Thurston Arthritis Research Center, Chapel Hill, North Carolina.
Clin Immunol Immunopathol. 1994 Oct;73(1):132-41. doi: 10.1006/clin.1994.1179.
The Su autoantigen was characterized biochemically using human and murine autoimmune sera and the clinical significance of anti-Su antibodies was studied in 236 Japanese and 160 American patients with systemic rheumatic diseases. Anti-Su in immunodiffusion (ID) was strongly associated with immunoprecipitation of one or more 100- to 102-kDa proteins by MRL/lpr mouse sera (27/32 of ID positive vs 4/20 of ID negative, P = 0.000016), and all four human anti-Su reference sera immunoprecipitated the 100/102-kDa protein(s). In addition, all sera immunoprecipitated a less efficiently labeled approximately 200-kDa protein that comigrated on sucrose density gradients with the 100/102-kDa proteins. Based on these data, a complex of the 100/102-kDa and 200-kDa proteins is likely to be the main target of anti-Su antibodies. Three of four anti-Su monospecific sera were negative for immunofluorescent antinuclear antibodies (ANA), suggesting anti-Su antibodies may be associated with a negative ANA in some cases. Autoantibodies to Su were detected frequently by immunoprecipitation in systemic lupus erythematosus (17-21%), scleroderma (13-20%), and overlap syndrome (22-40%) and were associated with autoantibodies to Ku.
利用人和小鼠自身免疫血清对Su自身抗原进行了生化特性分析，并在236例日本和160例美国系统性风湿疾病患者中研究了抗Su抗体的临床意义。免疫扩散法（ID）检测的抗Su与MRL/lpr小鼠血清对一种或多种100至102 kDa蛋白的免疫沉淀密切相关（ID阳性者27/32，ID阴性者4/20，P = 0.000016），并且所有四种人抗Su参考血清均能免疫沉淀100/102 kDa蛋白。此外，所有血清均能免疫沉淀一种标记效率较低的约200 kDa蛋白，该蛋白在蔗糖密度梯度上与100/102 kDa蛋白迁移率相同。基于这些数据，100/102 kDa和200 kDa蛋白的复合物可能是抗Su抗体的主要靶标。四种抗Su单特异性血清中有三种抗核抗体（ANA）免疫荧光检测为阴性，提示抗Su抗体在某些情况下可能与ANA阴性有关。系统性红斑狼疮（17 - 21%）、硬皮病（13 - 20%）和重叠综合征（22 - 40%）患者中通过免疫沉淀法经常检测到抗Su自身抗体，且与抗Ku自身抗体有关。
