Yamano K, Araki K, Sekikawa K, Inui Y
National Research Institute of Aquaculture, Mie, Japan.
Dev Genet. 1994;15(4):378-82. doi: 10.1002/dvg.1020150409.
Two distinct cDNAs encoding thyroid hormone receptors (THRs) were cloned from a lambda gt10 library prepared from the whole bodies of metamorphosing flounder larvae (Paralichthys olivaceus). Deduced amino acid sequences of the two isolated cDNAs shared 96% and 92% homologies in their DNA- and hormone-binding domains, respectively. These were highly conserved when compared to THRs for other vertebrates: 88-96% in the DNA-binding domain and 84-94% in the hormone-binding domain. Other receptors in the nuclear receptor family showed lower homologies than those of THRs. Both THRs for the flounder had higher homologies with the alpha-type THRs of other vertebrates than with the beta-type. Thus, the two THRs for flounder were designated as fTHR alpha A and fTHR alpha B.
从处于变态期的牙鲆幼鱼（Paralichthys olivaceus）整体制备的λgt10文库中克隆出了两个编码甲状腺激素受体（THRs）的不同cDNA。两个分离出的cDNA推导的氨基酸序列在其DNA结合域和激素结合域分别具有96%和92%的同源性。与其他脊椎动物的THRs相比，这些同源性高度保守：DNA结合域为88 - 96%，激素结合域为84 - 94%。核受体家族中的其他受体显示出比THRs更低的同源性。牙鲆的两种THRs与其他脊椎动物的α型THRs的同源性高于与β型的同源性。因此，牙鲆的两种THRs被命名为fTHRαA和fTHRαB。
