一种使用4-甲基伞形酮基-N-乙酰-D-氨基葡萄糖苷作为底物在交叉免疫电泳和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳凝胶中检测N-乙酰氨基葡萄糖苷酶型几丁质酶活性的快速方法。

A rapid method for detection of N-acetylglucosaminidase-type chitinase activity in crossed immunoelectrophoresis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels using 4-methylumbelliferyl-N-acetyl-D-glucosaminide as substrate.

作者信息

Chen K S, Lee K K, Chen H C

机构信息

Department of Marine Food Science, National Taiwan Ocean University, Keelung, Republic of China.

出版信息

Electrophoresis. 1994 May;15(5):662-5. doi: 10.1002/elps.1150150193.

DOI:10.1002/elps.1150150193

PMID:7925246

Abstract

A method for the detection of N-acetylglucosaminidase (GlcNAcase) activity has been developed by using 4-methyl-umbelliferyl-N-acetyl-D-glucosaminide (4-MU-GlcNAc) as substrate in crossed immunoelectrophoresis (CIE) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels. Visualization of the reaction with a 366 nm ultra-violet light is possible in approximately 30 min. The method is fast and sensitive in comparison with previous methods. The same band as in SDS-PAGE, showing both GlcNAcase and chitinase activity, was found in the present study; we therefore conclude that this method is also useful in a GlcNAcase-type chitinase assay.

摘要

通过使用4-甲基伞形酮基-N-乙酰-D-葡糖胺（4-MU-GlcNAc）作为底物，在交叉免疫电泳（CIE）和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）凝胶中，开发了一种检测N-乙酰葡糖胺酶（GlcNAcase）活性的方法。在约30分钟内，用366nm紫外线即可观察到反应。与先前的方法相比，该方法快速且灵敏。在本研究中发现了与SDS-PAGE中相同的条带，该条带同时显示出GlcNAcase和几丁质酶活性；因此，我们得出结论，该方法也可用于GlcNAcase型几丁质酶分析。