Morales-Ramírez P, Mendiola-Cruz M T, Vallarino-Kelly T, Rodríguez-Reyes R
Instituto Nacional de Investigaciones Nucleares, Lomas Barrilaco, México, D.F., Mexico.
Environ Mol Mutagen. 1994;24(2):89-95. doi: 10.1002/em.2850240203.
Sister chromatid exchange (SCE) induction by gamma rays was determined in spermatogonia irradiated before or after BrdU incorporation. Furthermore, the comparison of responses obtained in spermatogonia, bone marrow and salivary gland cells was carried out in the cells irradiated after BrdU incorporation, a condition which permits a higher SCE induction. Results indicate that gamma ray exposure of spermatogonia could induce a significant increase in SCE frequency with doses as low as 0.27 Gy, either before or after BrdU incorporation. However, the increase caused by radiation exposure after BrdU incorporation in spermatogonia was nearly three times lower than that obtained in both bone marrow and salivary gland cells. These data suggest that spermatogonia are either more efficient in repairing the gamma ray-induced lesions involved in SCE production or that these cells are less prone to the induction of such lesions.
在BrdU掺入之前或之后照射精原细胞，测定γ射线诱导的姐妹染色单体交换（SCE）。此外，在BrdU掺入后照射的细胞中，对精原细胞、骨髓细胞和唾液腺细胞的反应进行了比较，这种条件下SCE诱导率更高。结果表明，无论在BrdU掺入之前还是之后，精原细胞受到γ射线照射，剂量低至0.27 Gy时都能显著增加SCE频率。然而，BrdU掺入后精原细胞受到辐射照射引起的增加比骨髓细胞和唾液腺细胞低近三倍。这些数据表明，精原细胞要么在修复参与SCE产生的γ射线诱导损伤方面更有效，要么这些细胞更不容易受到此类损伤的诱导。
