de Bruijn M F, Slieker W A, van der Loo J C, Voerman J S, van Ewijk W, Leenen P J
Department of Immunology, Erasmus University, Rotterdam, The Netherlands.
Eur J Immunol. 1994 Oct;24(10):2279-84. doi: 10.1002/eji.1830241003.
The characterization of early branch points in the differentiation of leukocytes requires identification of precursor cells in the bone marrow. Recently, we produced two monoclonal antibodies, ER-MP12 and ER-MP20, which in two-color flow-cytometric analysis divide the murine bone marrow into six defined subsets. Here we show, using fluorescence-activated cell sorting followed by macrophage colony-stimulating factor-stimulated culture in soft agar, that precursors of the mononuclear phagocyte system reside only within the ER-MP12hi20-, ER-MP12+20+ and ER-MP12-20hi bone marrow subsets. Together, these subsets comprise 15% of nucleated bone marrow cells. Furthermore, we provide evidence that the macrophage precursors present in these subsets represent successive stages in a maturation sequence where the most immature ER-MP12hi20- cells develop via the ER-MP12+20+ stage into ER-MP12-20hi monocytes.
对白细胞分化早期分支点的特征描述需要鉴定骨髓中的前体细胞。最近，我们制备了两种单克隆抗体，ER-MP12和ER-MP20，在双色流式细胞术分析中，它们将小鼠骨髓分为六个明确的亚群。在此我们表明，通过荧光激活细胞分选，随后在软琼脂中进行巨噬细胞集落刺激因子刺激培养，单核吞噬细胞系统的前体细胞仅存在于ER-MP12hi20-、ER-MP12+20+和ER-MP12-20hi骨髓亚群中。这些亚群共同构成了有核骨髓细胞的15%。此外，我们提供证据表明，这些亚群中存在的巨噬细胞前体代表了成熟序列中的连续阶段，其中最不成熟的ER-MP12hi20-细胞通过ER-MP12+20+阶段发育为ER-MP12-20hi单核细胞。
