Keogh B, Pauly J, Tritsch G, Mittelman A, Murphy G P
J Surg Oncol. 1976;8(5):417-24. doi: 10.1002/jso.2930080508.
Adenosine-aminohydrolase activity in the red blood cells and lymphocytes of transplant patients were assayed by continuous flow analysis. This enzyme, which has been implicated in the ability of lymphocytes to mount an immune response, was evaluated in renal allografted patients immunosuppressed by azothiprine and methylprednisone. Red cell adenosine-aminohydrolase activity was depressed in all patients when compared to nontreated health controls. Adenosine aminohydrolase activity was raised in the lymphocytes of the renal allografted patients. Renal transplant patients' in vitro lymphocyte reactivity to antigens, mitogens, or skin testing to specific antigens, confirmed the depressive effects of immunosuppression observed in vivo. We conclude from these studies that red cell adenosine-aminohydrolase activity may be a more sensitive index of the state of immunoresponsiveness than the corresponding enzyme activity in the lymphocyte.
采用连续流动分析法检测移植患者红细胞和淋巴细胞中的腺苷氨基水解酶活性。这种酶与淋巴细胞产生免疫反应的能力有关，在接受硫唑嘌呤和甲基强的松龙免疫抑制治疗的同种异体肾移植患者中对其进行了评估。与未经治疗的健康对照组相比，所有患者的红细胞腺苷氨基水解酶活性均降低。同种异体肾移植患者淋巴细胞中的腺苷氨基水解酶活性升高。肾移植患者体外淋巴细胞对抗原、有丝分裂原的反应性或对特定抗原的皮肤试验，证实了体内观察到的免疫抑制的抑制作用。我们从这些研究中得出结论，红细胞腺苷氨基水解酶活性可能比淋巴细胞中相应的酶活性更能敏感地反映免疫反应状态。
