Laurent-Cadoret V, Guillou F, Combarnous Y
Station de Physiologie de la Reproduction des Mammifères Domestiques PRMD, INRA/CNRS, URA 1291, Nouzilly, France.
FEBS Lett. 1994 Sep 19;352(1):19-23. doi: 10.1016/0014-5793(94)00906-6.
Tissue-type plasminogen activator (tPA) secretion is a specific response of Sertoli cells to follicle-stimulating hormone (FSH), which is lower after preincubation of the cells with low FSH concentrations because of FSH receptor/Gs protein uncoupling. In this report, we present evidence that this desensitization induced by the lowest FSH concentrations is suppressed by specific peptidic inhibitors of endogenous PKA and PKC in permeabilized Sertoli cells. In contrast, desensitization promoted by slightly higher FSH concentrations is not mediated through PKA or PKC activation but is dependent on protein neosynthesis.
组织型纤溶酶原激活物（tPA）的分泌是支持细胞对促卵泡激素（FSH）的特异性反应，由于FSH受体与Gs蛋白解偶联，在细胞用低浓度FSH预孵育后，该反应会降低。在本报告中，我们提供证据表明，在通透的支持细胞中，内源性PKA和PKC的特异性肽抑制剂可抑制最低FSH浓度诱导的这种脱敏作用。相反，稍高浓度FSH促进的脱敏作用不是通过PKA或PKC激活介导的，而是依赖于蛋白质的新合成。
