Jirsakova V, Reiss-Husson F
Centre de Génétique Moléculaire, Gif sur Yvette, France.
FEBS Lett. 1994 Oct 17;353(2):151-4. doi: 10.1016/0014-5793(94)01033-1.
The core light-harvesting complex B875 from Rubrivivax gelatinosus has been reconstituted from its subunit form B820 with hydroxyspheroidene, the carotenoid which is bound to native B875 antenna. Other carotenoids which are chemically similar to hydroxyspheroidene (spheroidene, spheroidenone, neurosporene and spirilloxanthin) gave only low levels of partial reconstitution. Absorption and circular dichroism spectra of the hydroxyspheroidene-containing, reconstituted B875 were identical with those of original B875 antenna isolated directly from chromatophores, indicating that the two hydroxyspheroidene molecules bind to their native sites within the (alpha beta)Bchl2 subunit during the reconstitution process. These observations point to a structural role for this carotenoid in determining the architecture of Rv. gelatinosus B875 antenna.
嗜红红假单胞菌的核心捕光复合物B875已由其亚基形式B820与羟基球形烯重建而成，羟基球形烯是一种与天然B875天线结合的类胡萝卜素。其他与羟基球形烯化学性质相似的类胡萝卜素（球形烯、球形烯酮、神经孢烯和螺菌黄素）仅产生低水平的部分重建。含羟基球形烯的重建B875的吸收光谱和圆二色光谱与直接从载色体分离的原始B875天线的光谱相同，这表明在重建过程中，两个羟基球形烯分子与其在（αβ）Bchl2亚基内的天然位点结合。这些观察结果表明，这种类胡萝卜素在决定嗜红红假单胞菌B875天线的结构方面具有结构作用。
