Stavenhagen J B, Zakian V A
Fred Hutchinson Cancer Research Center, Seattle, Washington 98104.
Genes Dev. 1994 Jun 15;8(12):1411-22. doi: 10.1101/gad.8.12.1411.
Telomeric position effect (TPE) refers to the ability of telomeres to repress the transcription of genes in their vicinity. Internal stretches of C1-3A DNA, the sequence found at Saccharomyces telomeres, also repressed transcription of nearby genes. This repression, hereafter called C1-3A-based silencing, was observed at several chromosomal loci, including on a circular chromosome. The magnitude of C1-3A-based silencing was increased by both proximity to a telomere and increased length of the C1-3A tracts. C1-3A-based silencing was affected by many of the same genes and conditions that influence TPE and acted in an orientation-independent manner. Thus, in yeast, an expanded array of a simple repetitive DNA, C1-3A, is sufficient to promote the assembly of a transcriptionally silent chromosomal domain.
端粒位置效应（TPE）是指端粒抑制其附近基因转录的能力。酿酒酵母端粒处的C1-3A DNA内部序列也能抑制附近基因的转录。这种抑制作用，以下称为基于C1-3A的沉默，在几个染色体位点都有观察到，包括在环状染色体上。基于C1-3A的沉默程度会因靠近端粒以及C1-3A序列长度增加而增强。基于C1-3A的沉默受到许多影响TPE的相同基因和条件的影响，并且以不依赖方向的方式起作用。因此，在酵母中，简单重复DNA C1-3A的扩展阵列足以促进转录沉默染色体结构域的组装。
