Hamada T, Kodama H, Nishimura M, Iba K
Department of Biology, Faculty of Science, Kyushu University, Fukuoka, Japan.
Gene. 1994 Sep 30;147(2):293-4. doi: 10.1016/0378-1119(94)90084-1.
A cDNA was isolated from a tobacco (Nicotiana tabacum cv. SR1) leaf cDNA library using, as a hybridization probe, a cDNA fragment from the gene (fad7) encoding Arabidopsis thaliana chloroplast omega-3 fatty acid (FA) desaturase. The deduced 379-amino-acid (aa) sequence has 67-71% identity to those deduced from the previously described genes encoding the omega-3 FA desaturases of A. thaliana. The absence of an N-terminal extension transit peptide in the deduced aa sequence of the cDNA clone and the accumulation pattern of the mRNA corresponding to this cDNA in leaf and root tissues indicate that the isolated cDNA encodes a tobacco microsomal omega-3 FA desaturase.
利用编码拟南芥叶绿体ω-3脂肪酸（FA）去饱和酶的基因（fad7）的cDNA片段作为杂交探针，从烟草（Nicotiana tabacum cv. SR1）叶片cDNA文库中分离出一个cDNA。推导的379个氨基酸（aa）序列与先前描述的编码拟南芥ω-3 FA去饱和酶的基因推导的序列具有67%-71%的同一性。cDNA克隆推导的aa序列中没有N端延伸转运肽，以及该cDNA对应的mRNA在叶和根组织中的积累模式表明，分离出的cDNA编码一种烟草微粒体ω-3 FA去饱和酶。
