Ives J T, Gesteland R F, Stockham T G
University of Utah, Department of Human Genetics, Salt Lake City 84112.
IEEE Trans Biomed Eng. 1994 Jun;41(6):509-19. doi: 10.1109/10.293238.
An automated reader for electrophoresis based DNA sequencing methods is described that provides fast and accurate sequence determination. Digitized sequencing lanes are processed with homomorphic blind deconvolution in preparation for peak detection, interlane alignment, peak refinement and base calling. Initial reads from direct blot sequencing films have error rates of about 1% at the rate of 5 nucleotides/s. Typical read lengths are 500-600 nucleotides. The described reader is a significant improvement over existing readers and could be an essential component in the sequencing efforts of the Human Genome Project.
本文描述了一种用于基于电泳的DNA测序方法的自动读取器，它能实现快速且准确的序列测定。数字化的测序泳道通过同态盲反卷积进行处理，以准备进行峰检测、泳道间比对、峰细化和碱基识别。直接印迹测序胶片的初始读取在每秒5个核苷酸的速率下错误率约为1%。典型的读取长度为500 - 600个核苷酸。所描述的读取器相较于现有读取器有显著改进，可能成为人类基因组计划测序工作中的重要组成部分。
