由转基因抗原呈递细胞表达的单一分枝杆菌蛋白（hsp 65）可使小鼠接种预防结核病的疫苗。
A single mycobacterial protein (hsp 65) expressed by a transgenic antigen-presenting cell vaccinates mice against tuberculosis.
作者信息
Silva C L, Lowrie D B
机构信息
Department of Parasitology, Microbiology and Immunology, School of Medicine of Ribeirão Preto, University of São Paolo, Brazil.
出版信息
Immunology. 1994 Jun;82(2):244-8.
Abstract
We used a retroviral shuttle vector [pZIPNeoSV(X)] to transfect a monocyte-like murine tumour cell line (J774.G8) with the Mycobacterium leprae gene encoding heat-shock protein (hsp) 65. The antigen was expressed and presented on the surface of the transfected cell in association with major histocompatibility complex (MHC) class I and class II for recognition by T cells from specifically sensitized mice. We show here that when these transfected cells were used as a vaccine and introduced parentally into syngeneic (BALB/c) mice they conferred a remarkably high degree of protective immunity against subsequent challenge with either M. bovis bacillus Calmette-Guérin (BCG) or M. tuberculosis H37Rv.
摘要
我们使用逆转录病毒穿梭载体[pZIPNeoSV(X)]，将编码热休克蛋白（hsp）65的麻风分枝杆菌基因转染至单核细胞样小鼠肿瘤细胞系（J774.G8）。该抗原得以表达，并与主要组织相容性复合体（MHC）I类和II类分子一起呈递于转染细胞表面，以供来自特异性致敏小鼠的T细胞识别。我们在此表明，当这些转染细胞用作疫苗并经腹腔注射引入同基因（BALB/c）小鼠时，它们能对随后用牛型结核分枝杆菌卡介苗（BCG）或结核分枝杆菌H37Rv进行的攻击赋予极高程度的保护性免疫。
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