Yashima K, Nakamori S, Murakami Y, Yamaguchi A, Hayashi K, Ishikawa O, Konishi Y, Sekiya T
Oncogene Division, National Cancer Center Research Institute, Tokyo, Japan.
Int J Cancer. 1994 Oct 1;59(1):43-7. doi: 10.1002/ijc.2910590110.
We analyzed the adenomatous polyposis coli (APC) tumor-suppressor gene as one of the possible genes mutated in human pancreatic carcinomas. DNAs from 39 surgical specimens were subjected to single-strand conformation polymorphism analysis of polymerase chain reaction products and the mutation cluster region (MCR) of the gene was examined. We also examined the same region of DNAs from 27 surgical specimens of sporadic colon carcinomas and detected mutations in 11 carcinomas (41%). This mutation frequency in colon carcinomas was similar to those reported previously. Using this system, we detected a mutated APC gene in one of 39 pancreatic carcinomas. The results indicated that mutation of the MCR in the APC gene is involved in genesis of some of human pancreatic carcinomas, but its frequency is much lower than in colorectal carcinomas.
我们分析了腺瘤性息肉病 coli（APC）肿瘤抑制基因，将其作为人类胰腺癌中可能发生突变的基因之一。对 39 个手术标本的 DNA 进行聚合酶链反应产物的单链构象多态性分析，并检测该基因的突变簇区域（MCR）。我们还检测了 27 个散发性结肠癌手术标本 DNA 的相同区域，在 11 个癌组织中检测到突变（41%）。结肠癌中的这种突变频率与先前报道的相似。利用该系统，我们在 39 个胰腺癌标本中的 1 个中检测到 APC 基因发生突变。结果表明，APC 基因 MCR 的突变参与了部分人类胰腺癌的发生，但其频率远低于结直肠癌。
