Agorastos T, Vakiani A, Papaloucas A
B' University Clinic of Obstetrics and Gynecology, Aristotelian University of Thessaloniki, Greece.
Int J Gynecol Pathol. 1994 Jul;13(3):211-9. doi: 10.1097/00004347-199407000-00004.
Minimal deviation adenocarcinoma (MDA) of the uterine cervix is an extremely well-differentiated adenocarcinoma arising from the endocervix, with minimal morphological deviation from normal endocervical columnar epithelium. We performed DNA cytometric investigation using DNA image analysis in three cases of MDA. For the diagnosis of DNA aneuploidy, we used a combined method of single cell and stemline interpretation of DNA histograms. The most significant finding is that DNA image analysis of all three cases of MDA revealed a hypodiploid stemline DNA value in the histograms of the measured cells and consequently the diagnosis of DNA aneuploidy (DNA index: 0.63, 0.88, and 0.63, respectively). On the basis of our results, we believe that DNA image analysis is useful as a diagnostic aid in doubtful cases of MDA. We find MDA is an aneuploid tumor with a hypodiploid stem cell subpopulation.
子宫颈微小偏离腺癌（MDA）是一种起源于子宫颈管内膜的高分化腺癌，其形态与正常子宫颈管柱状上皮仅有微小差异。我们对3例MDA患者进行了DNA图像分析的DNA细胞计量学研究。对于DNA非整倍体的诊断，我们采用了单细胞和干系解释DNA直方图的联合方法。最显著的发现是，所有3例MDA患者的DNA图像分析均显示，在所测细胞的直方图中存在亚二倍体干系DNA值，因此诊断为DNA非整倍体（DNA指数分别为0.63、0.88和0.63）。基于我们的研究结果，我们认为DNA图像分析在MDA疑难病例的诊断中可作为一种辅助诊断手段。我们发现MDA是一种具有亚二倍体干细胞亚群的非整倍体肿瘤。
