Chen J Y, Harrison T J, Tsuei D J, Hsu T Y, Zuckerman A J, Chan T S, Yang C S
Graduate Institute of Microbiology, College of Medicine, National Taiwan University, Taipei, Republic of China.
Intervirology. 1994;37(1):41-6. doi: 10.1159/000150355.
A hepatocellular carcinoma cell line, HCC36, was established from an adult HBV carrier in Taiwan. From Southern blot analysis, there were at least four sites of integration of HBV DNA, and no viral replicative intermediates were detected. A genomic library was constructed from HCC36 DNA, and two phage clones, designated lambda 36A and lambda 36B, were shown to contain HBV DNA and flanking cellular sequences. In lambda 36A, HBV DNA sequences were quite conserved, and 7.4% base variation was detected. The viral sequences in lambda 36A and lambda 36B differed in only four bases, in addition to the microdeletion and -insertion observed in lambda 36B. The flanking cellular sequences identified in lambda 36A were human Alu sequences and in lambda 36B satellite sequences.
肝癌细胞系HCC36是从台湾一名成年乙肝病毒携带者体内建立的。通过Southern印迹分析，发现乙肝病毒DNA至少有四个整合位点，且未检测到病毒复制中间体。用HCC36 DNA构建了一个基因组文库，两个噬菌体克隆，命名为λ36A和λ36B，被证明含有乙肝病毒DNA和侧翼细胞序列。在λ36A中，乙肝病毒DNA序列相当保守，检测到7.4%的碱基变异。除了在λ36B中观察到的微缺失和插入外，λ36A和λ36B中的病毒序列仅在四个碱基上有所不同。在λ36A中鉴定出的侧翼细胞序列是人类Alu序列，在λ36B中是卫星序列。
