血红蛋白向稳定四聚体的定量转化。

Quantitative transformation of hemoglobin into stable tetramers.

作者信息

Benesch R E, Kwong S

机构信息

Department of Biochemistry & Molecular Biophysics, College of Physicians & Surgeons of Columbia University New York, NY 10032.

出版信息

Hemoglobin. 1994 May;18(3):185-92. doi: 10.3109/03630269409043618.

DOI:10.3109/03630269409043618

PMID:7928375

Abstract

A method is described for the preparation of human or bovine hemoglobin with a covalent bridge, formed by bispyridoxal-tetraphosphate, between the beta chains. The yield is 95% of the total hemoglobin. The location of the two molecules of bispyridoxal-tetraphosphate in the tetramer has been established. The functional properties of the cross-linked hemoglobin, its stability, and particularly, the simplicity of the method for its preparation, make it a promising candidate for an acellular blood substitute.

摘要

描述了一种制备人或牛血红蛋白的方法，该血红蛋白在β链之间具有由双磷酸吡哆醛形成的共价桥。产率为总血红蛋白的95%。已确定双磷酸吡哆醛的两个分子在四聚体中的位置。交联血红蛋白的功能特性、其稳定性，特别是其制备方法的简便性，使其成为一种有前景的无细胞血液替代品候选物。