Molto M D, Martinez-Sebastian M J, De Frutos R
Departament de Genètica, Facultat de Ciències Biológiques, Universitat de València, Spain.
Hereditas. 1994;120(3):217-23. doi: 10.1111/j.1601-5223.1994.00217.x.
A Southern analysis of genomic DNA using Drosophila melanogaster probes for the major heat shock protein genes (Hsp82, Hsp70, Hsps encoding small proteins) was made to study the phylogenetic relationships between three Drosophila species belonging to the obscura group (D. subobscura, D. guanche, and D. madeirensis). The phylogenetic trees showed that D. madeirensis and D. subobscura are the most closely related species in the triad, while D. guanche is the most distantly related one. As in other Drosophila species, Hsp82 is a single copy gene in D. subobscura, D. guanche, and D. madeirensis, while Hsp70 and Hsps, which encode small proteins, are genic families. At least four sequences homologous to D. melanogaster Hsp70 were found in the obscura group species. These species have sequences which showed similarity with the four small Hsps of D. melanogaster.
使用黑腹果蝇主要热休克蛋白基因（Hsp82、Hsp70、编码小蛋白的Hsps）的探针，对基因组DNA进行Southern分析，以研究属于obscura组的三种果蝇（D. subobscura、D. guanche和D. madeirensis）之间的系统发育关系。系统发育树显示，在这三种果蝇中，D. madeirensis和D. subobscura是关系最密切的物种，而D. guanche是关系最疏远的物种。与其他果蝇物种一样，Hsp82在D. subobscura、D. guanche和D. madeirensis中是单拷贝基因，而编码小蛋白的Hsp70和Hsps是基因家族。在obscura组物种中发现了至少四个与黑腹果蝇Hsp70同源的序列。这些物种的序列与黑腹果蝇的四种小Hsps显示出相似性。
