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本文引用的文献

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Deletion between directly repeated DNA sequences measured in extracts of bacteriophage T7-infected Escherichia coli.在噬菌体T7感染的大肠杆菌提取物中测量的直接重复DNA序列之间的缺失。
J Biol Chem. 1993 Apr 15;268(11):7721-7.
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A sister-strand exchange mechanism for recA-independent deletion of repeated DNA sequences in Escherichia coli.大肠杆菌中recA非依赖型重复DNA序列缺失的姐妹链交换机制。
Genetics. 1993 Nov;135(3):631-42. doi: 10.1093/genetics/135.3.631.
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Mechanisms of illegitimate recombination.异常重组的机制。
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Repair and misrepair of site-specific DNA double-strand breaks by human cell extracts.人类细胞提取物对位点特异性DNA双链断裂的修复与错修复
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Double-strand breaks in plasmid DNA and the induction of deletions.质粒DNA中的双链断裂与缺失的诱导
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Mutagenesis of bacteriophage T7 in vitro by incorporation of O6-methylguanine during DNA synthesis.在DNA合成过程中通过掺入O6-甲基鸟嘌呤对噬菌体T7进行体外诱变。
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Model for homologous recombination during transfer of DNA into mouse L cells: role for DNA ends in the recombination process.DNA转入小鼠L细胞过程中的同源重组模型：DNA末端在重组过程中的作用。
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On the formation of spontaneous deletions: the importance of short sequence homologies in the generation of large deletions.关于自发缺失的形成：短序列同源性在大缺失产生中的重要性。
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双链断裂刺激T7 DNA中同向重复序列间的缺失。

Deletion between direct repeats in T7 DNA stimulated by double-strand breaks.

作者信息

Kong D, Masker W

机构信息

Department of Biochemistry and Molecular Biology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140.

出版信息

J Bacteriol. 1994 Oct;176(19):5904-11. doi: 10.1128/jb.176.19.5904-5911.1994.

DOI:10.1128/jb.176.19.5904-5911.1994
PMID:7928950
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC196806/
Abstract

An in vitro system based on extracts of Escherichia coli infected with bacteriophage T7 was used to study genetic deletions between directly repeated sequences. The frequency of deletion was highest under conditions in which the DNA was actively replicating. Deletion frequency increased markedly with the length of the direct repeat both in vitro and in vivo. When a T7 gene was interrupted by 93 bp of nonsense sequence flanked by 20-bp direct repeats, the region between the repeats was deleted in about 1 out of every 1,600 genomes during each round of replication. Very similar values were found for deletion frequency in vivo and in vitro. The deletion frequency was essentially unaffected by a recA mutation in the host. When a double-strand break was placed between the repeats, repair of this strand break was often accompanied by the deletion of the DNA between the direct repeats, suggesting that break rejoining could contribute to deletion during in vitro DNA replication.

摘要

基于感染噬菌体T7的大肠杆菌提取物构建的体外系统，用于研究直接重复序列之间的基因缺失。在DNA进行活跃复制的条件下，缺失频率最高。在体外和体内，缺失频率均随直接重复序列长度的增加而显著升高。当一个T7基因被一段93 bp的无义序列中断，该无义序列两侧为20 bp的直接重复序列时，在每一轮复制过程中，每1600个基因组中约有1个基因组的重复序列之间的区域会被删除。在体内和体外发现的缺失频率非常相似。宿主中的recA突变对缺失频率基本没有影响。当在重复序列之间产生双链断裂时，该链断裂的修复常常伴随着直接重复序列之间DNA的缺失，这表明断裂重接可能在体外DNA复制过程中导致缺失。