AnfA潜在金属结合位点的氨基酸替换对棕色固氮菌中anfH启动子表达的影响。
Effect of amino acid substitutions in a potential metal-binding site of AnfA on expression from the anfH promoter in Azotobacter vinelandii.
作者信息
Premakumar R, Loveless T M, Bishop P E
机构信息
Agricultural Research Service, U.S. Department of Agriculture, Raleigh, North Carolina.
出版信息
J Bacteriol. 1994 Oct;176(19):6139-42. doi: 10.1128/jb.176.19.6139-6142.1994.
Abstract
AnfA, an activator required for transcription of the structural genes encoding nitrogenase 3 (anfHDGK) in Azotobacter vinelandii, has a potential metal-binding site [(S19)H(C21)FTGE(C26)R] in its N terminus. Growth studies and expression of an anfH-lacZ fusion in mutants containing amino acid substitutions in this site indicate that Ser-19 is not required for AnfA activity whereas Cys-21 and Cys-26 are required. Residual expression of the anfH-lacZ fusion in AnfA- mutants was found to be due to activation by VnfA, the activator required for expression of genes encoding nitrogenase 2.
摘要
AnfA是维涅兰德固氮菌中编码固氮酶3（anfHDGK）的结构基因转录所需的激活因子，其N端有一个潜在的金属结合位点[（S19）H（C21）FTGE（C26）R]。对该位点含有氨基酸取代的突变体进行生长研究以及anfH-lacZ融合蛋白的表达分析，结果表明Ser-19对AnfA活性不是必需的，而Cys-21和Cys-26是必需的。发现AnfA-突变体中anfH-lacZ融合蛋白的残留表达是由于VnfA的激活，VnfA是编码固氮酶2的基因表达所需的激活因子。
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Effect of amino acid substitutions in a potential metal-binding site of AnfA on expression from the anfH promoter in Azotobacter vinelandii.AnfA潜在金属结合位点的氨基酸替换对棕色固氮菌中anfH启动子表达的影响。
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