Sica G, Iacopino F, Robustelli della Cuna G, Marchetti P, Marini L
Istituto di Istologia ed Embriologia, Università Cattolica del Sacro Cuore, Roma, Italy.
J Cancer Res Clin Oncol. 1994;120(10):605-9. doi: 10.1007/BF01212815.
Both tamoxifen and medroxyprogesterone acetate have a direct antitumor effect and are widely used in breast cancer therapy. Luteinizing-hormone-releasing hormone analogs inhibit the growth of breast cancer cells and could represent an alternative treatment for patients affected by breast cancer. Our study was carried out to investigate the effect of leuprorelin (TAP-144) alone or combined with tamoxifen or medroxyprogesterone acetate in human breast cancer cells. Ineffective when used in the absence of estrogens, TAP-144 inhibited the estrogen-stimulated growth of MCF-7, CG-5 and ZR-75-1 cells cultured in medium supplemented with charcoal-treated serum. The growth of estrogen-unresponsive MDA-MB-231 cells was not affected by TAP-144. The combination of TAP-144 with tamoxifen in CG-5 cells did not determine any enhancement of inhibition of cell growth, whereas in both CG-5 and MCF-7 cells, when 1 microM TAP-144 was associated with 0.1 microM medroxyprogesterone acetate, cell growth inhibition was increased, resulting in a subadditive effect. Progesterone receptor levels of CG-5 cells were significantly increased by TAP-144 in the presence of 17 beta-estradiol with respect to those present in control and 17 beta-estradiol-treated cells.
他莫昔芬和醋酸甲羟孕酮都具有直接抗肿瘤作用，广泛应用于乳腺癌治疗。促黄体激素释放激素类似物可抑制乳腺癌细胞生长，可能为乳腺癌患者提供一种替代治疗方法。我们开展这项研究以调查亮丙瑞林（TAP - 144）单独使用或与他莫昔芬或醋酸甲羟孕酮联合使用对人乳腺癌细胞的影响。在缺乏雌激素时无效，TAP - 144抑制了在添加经活性炭处理血清的培养基中培养的MCF - 7、CG - 5和ZR - 75 - 1细胞的雌激素刺激生长。雌激素无反应的MDA - MB - 231细胞的生长不受TAP - 144影响。在CG - 5细胞中，TAP - 144与他莫昔芬联合使用并未增强对细胞生长的抑制作用，而在CG - 5和MCF - 7细胞中，当1微摩尔TAP - 144与0.1微摩尔醋酸甲羟孕酮联合使用时，细胞生长抑制作用增强，产生次加性效应。与对照细胞和17β - 雌二醇处理的细胞相比，在17β - 雌二醇存在下，TAP - 144使CG - 5细胞的孕酮受体水平显著升高。
