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层粘连蛋白在支持细胞与睾丸间质细胞共培养过程中形态发生级联反应中的作用。

Role of laminin in the morphogenetic cascade during coculture of Sertoli cells with peritubular cells.

作者信息

Tung P S, Fritz I B

机构信息

Banting and Best Department of Medical Research, C.H. Best Institute, University of Toronto, Ontario, Canada.

出版信息

J Cell Physiol. 1994 Oct;161(1):77-88. doi: 10.1002/jcp.1041610111.

DOI:10.1002/jcp.1041610111
PMID:7929611
Abstract

Observations summarized in this article demonstrate an essential role of laminin during the restructuring processes that occur during coculture of Sertoli cells with testicular peritubular cells. The data presented indicate that laminin becomes detectable on the free surfaces of Sertoli cells only after reaggregation of Sertoli cells begins, coincident with the initiation of repolarization at a specific stage of the morphogenetic cascade. We infer that laminin deposited at this time serves as a cohesion molecule that permits peritubular cells to come into close contact with Sertoli cells and subsequently to spread along the free surfaces of Sertoli cells. These conclusions and inferences are based on the following experiments. Cycloheximide-treated peritubular cells in culture in MEM containing cycloheximide readily attach to laminin-coated polystyrene surfaces. By contrast, added peritubular cells do not attach onto monolayers of Sertoli cells in monoculture or onto Sertoli cells plated on top of peritubular cells and maintained in coculture for periods of up to 48 h. In cocultures maintained for 6 days, however, labeled peritubular cells readily adhere to the free surfaces of reaggregated Sertoli cells. Laminin, but not fibronectin, appears on the free surfaces of the reaggregated Sertoli cells at this time, coinciding with the period of initial mound formation. The addition of antilaminin IgG, but not antifibronectin IgG, blocks the attachment of cycloheximide-treated peritubular cells to laminin-coated plates and also blocks the subsequent migration of peritubular cells required to form a monolayer. Similarly, anti-laminin IgG inhibits the attachment and spreading of labeled peritubular cells seeded on the free surfaces of reaggregated Sertoli cells in mounds generated during the morphogenetic cascade. We interpret the combined data to indicate that the appearance of laminin on the free surfaces of Sertoli cells is required to permit peritubular cells to adhere and subsequently to migrate on Sertoli cell surfaces, resulting in the formation of a tubule-like structure.

摘要

本文总结的观察结果表明,层粘连蛋白在支持细胞与睾丸生精小管周细胞共培养过程中发生的重组过程中起着重要作用。所呈现的数据表明,层粘连蛋白仅在支持细胞重新聚集开始后,即在形态发生级联反应的特定阶段复极化开始时,才在支持细胞的自由表面上被检测到。我们推断,此时沉积的层粘连蛋白作为一种黏附分子,使生精小管周细胞能够与支持细胞紧密接触,并随后沿支持细胞的自由表面扩散。这些结论和推断基于以下实验。在含有环己酰亚胺的MEM培养基中培养的经环己酰亚胺处理的生精小管周细胞很容易附着在层粘连蛋白包被的聚苯乙烯表面。相比之下,添加的生精小管周细胞不会附着在单培养的支持细胞单层上,也不会附着在接种于生精小管周细胞之上并维持共培养长达48小时的支持细胞上。然而,在维持6天的共培养中,标记的生精小管周细胞很容易附着在重新聚集的支持细胞的自由表面上。此时,层粘连蛋白而非纤连蛋白出现在重新聚集的支持细胞的自由表面上,这与最初形成小丘的时期一致。添加抗层粘连蛋白IgG而非抗纤连蛋白IgG可阻断经环己酰亚胺处理的生精小管周细胞与层粘连蛋白包被平板的附着,也可阻断随后形成单层所需的生精小管周细胞的迁移。同样,抗层粘连蛋白IgG抑制接种在形态发生级联反应过程中产生的小丘中重新聚集的支持细胞自由表面上的标记生精小管周细胞的附着和扩散。我们综合这些数据得出结论,支持细胞自由表面上层粘连蛋白的出现是生精小管周细胞附着并随后在支持细胞表面迁移从而形成管状结构所必需的。

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Role of laminin in the morphogenetic cascade during coculture of Sertoli cells with peritubular cells.层粘连蛋白在支持细胞与睾丸间质细胞共培养过程中形态发生级联反应中的作用。
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J Cell Biol. 1995 Nov;131(4):1105-21. doi: 10.1083/jcb.131.4.1105.