Miller D, Tang P Z, Skinner C, Lilford R
Department of Clinical Medicine, University of Leeds, UK.
Hum Reprod. 1994 May;9(5):864-9. doi: 10.1093/oxfordjournals.humrep.a138607.
The apparent decline in human male fertility and the concomitant increase in testicular pathology have prompted discussion of the underlying molecular mechanisms which may underpin these observations. While monitoring the expression of protamine-2 genes in the human ejaculate, we found a representative complement of sperm mRNAs following sequence-independent amplification of reverse-transcribed cDNAs with the polymerase chain reaction (RT-PCR). The revelation of unique sperm-derived PCR products using this method suggests that it should now be possible to investigate gene expression in human spermatogenesis by differential RNA fingerprinting of ejaculate spermatozoa. The identification of molecular markers and the corresponding genes associated with male infertility will be considerably enhanced by these investigations while obviating the requirement for invasive biopsy.
人类男性生育能力的明显下降以及随之而来的睾丸病理变化增加，引发了对可能支撑这些观察结果的潜在分子机制的讨论。在监测人类精液中鱼精蛋白-2基因的表达时，我们通过聚合酶链反应（RT-PCR）对逆转录的cDNA进行序列独立扩增后，发现了一组具有代表性的精子mRNA。使用这种方法揭示独特的精子来源PCR产物表明，现在应该可以通过对射出精子进行差异RNA指纹分析来研究人类精子发生过程中的基因表达。这些研究将大大增强对与男性不育相关的分子标记和相应基因的识别，同时避免了侵入性活检的需要。
