Moffett J R, Espey M G, Saito K, Namboodiri M A
Biology Department, Georgetown University, Washington, DC 20057-1028.
J Neuroimmunol. 1994 Oct;54(1-2):69-73. doi: 10.1016/0165-5728(94)90232-1.
Antibodies to quinolinic acid were utilized to study the cellular localization of this endogenous neurotoxin in the gerbil brain subsequent to systemic immune stimulation with pokeweed mitogen. Immunohistochemistry of carbodiimide fixed spleen revealed a dramatic increase in the number of quinolinic acid-positive cells in the red pulp in the immune-stimulated animals. Quinolinic acid immunoreactivity in the brain was observed in cells within the choroid plexus, vasculature and leptomeninges of the stimulated group only. No immunoreactivity was observed in brain parenchyma. These results are supportive of an immune system origin for the increases in quinolinic acid in CSF and brain during immune stimulation in a rodent model system.
利用抗喹啉酸抗体研究了在用商陆有丝分裂原进行全身免疫刺激后，这种内源性神经毒素在沙鼠脑中的细胞定位。碳二亚胺固定脾脏的免疫组织化学显示，免疫刺激动物的红髓中喹啉酸阳性细胞数量显著增加。仅在刺激组的脉络丛、脉管系统和软脑膜中的细胞中观察到脑中的喹啉酸免疫反应性。脑实质中未观察到免疫反应性。这些结果支持在啮齿动物模型系统的免疫刺激过程中，脑脊液和脑中喹啉酸增加的免疫系统起源。
