Alkan S, Akdis C, Towbin H
Department of Allergy/Immunology, Pharmaceuticals Research Division, Ciba-Geigy Ltd., Basel Switzerland.
J Immunoassay. 1994 Aug;15(3):217-38. doi: 10.1080/15321819408009574.
We have produced and characterized 4 mAbs to human IFN-gamma and established sensitive, non-radioactive immuno-assays. The first two assays use microtiter plates as the solid phase and enzymes or chemiluminescence (acridinium ester) for development. The use of chemiluminescence instead of peroxidase increased the sensitivity of the assay by a factor of about 75. The third and the fourth assays utilize polystyrene beads as the solid phase and enzymes or acridinium ester for development. The use of beads also increased the sensitivity of detection. The most sensitive IFN-gamma detection was achieved by the combination of bead with acridinium ester. In this configuration we were able to detect about 0.2 pg/ml IFN-gamma (1/250th of a unit). These chemiluminescent immunoassays (CLIA) appear to be more sensitive than existing ELISAs or radioimmunoassays and may find new application areas.
我们制备并鉴定了4种抗人γ干扰素单克隆抗体（mAbs），并建立了灵敏的非放射性免疫测定法。前两种测定法使用微量滴定板作为固相，用酶或化学发光（吖啶酯）进行显色。使用化学发光代替过氧化物酶使测定的灵敏度提高了约75倍。第三种和第四种测定法利用聚苯乙烯珠作为固相，用酶或吖啶酯进行显色。使用珠子也提高了检测的灵敏度。通过珠子与吖啶酯的组合实现了最灵敏的γ干扰素检测。在这种配置下，我们能够检测到约0.2 pg/ml的γ干扰素（1/250个单位）。这些化学发光免疫测定法（CLIA）似乎比现有的酶联免疫吸附测定法（ELISA）或放射免疫测定法更灵敏，可能会发现新的应用领域。
