Juvonen T, Parkkila S, Parkkila A K, Niemelä O, Lajunen L H, Kairaluoma M I, Perämäki P, Rajaniemi H
Department of Surgery, University of Oulu, Finland.
J Histochem Cytochem. 1994 Oct;42(10):1393-7. doi: 10.1177/42.10.7930522.
Acidification of bile is one of the factors that prevents calcium precipitation and thereby gallstone formation. Carbonic anhydrase II (CA II) has previously been shown to be one of the key factors in the human alimentary tract that regulates the acid-base balance. We demonstrated CA II expression in the human gallbladder epithelium using immunohistochemical techniques, elucidated the CA II content of the epithelium by digital image analysis of the immunohistochemically stained enzyme in samples from 16 patients undergoing cholecystectomy, and correlated the results with the calcium content of the gallstones. Nine patients had symptomatic gallstone disease and seven an acalculous, histologically normal gallbladder. The patients were classified into two groups on the basis of the calcium content of their gallstones: no gallstones or gallstones containing no calcium (Group 1) and gallstones with 2-87% calcium by weight (Group 2). The immunohistochemical techniques showed distinct epithelial CA II-positive staining in most of the gallbladder samples, but digital image analysis revealed distinct variations in staining intensity among them. The median staining intensity index was significantly higher in Group 1 (0.4463) than in Group 2 (0.2376; p = 0.0262). The results suggest that CA II is abundantly expressed in the normal gallbladder epithelium and that decreased expression may be associated with the formation of calcified gallstones. These findings are relevant to the pathogenesis of gallstone disease.
胆汁酸化是防止钙沉淀从而预防胆结石形成的因素之一。碳酸酐酶II（CA II）先前已被证明是人类消化道中调节酸碱平衡的关键因素之一。我们使用免疫组织化学技术证明了CA II在人胆囊上皮中的表达，通过对16例接受胆囊切除术患者的样本中免疫组织化学染色的酶进行数字图像分析，阐明了上皮细胞的CA II含量，并将结果与胆结石的钙含量相关联。9例患者患有有症状的胆结石疾病，7例为无结石、组织学正常的胆囊。根据胆结石的钙含量将患者分为两组：无胆结石或不含钙的胆结石（第1组）和含钙量为2 - 87%（重量）的胆结石（第2组）。免疫组织化学技术显示大多数胆囊样本中上皮CA II呈明显阳性染色，但数字图像分析显示它们之间染色强度存在明显差异。第1组的中位染色强度指数（0.4463）显著高于第2组（0.2376；p = 0.0262）。结果表明，CA II在正常胆囊上皮中大量表达，其表达降低可能与钙化胆结石的形成有关。这些发现与胆结石疾病的发病机制相关。
