Lake-Bakaar G, Bhat K, Govindarajan S
Department of Medicine, State University of New York Health Science Center at Brooklyn.
J Lab Clin Med. 1994 Oct;124(4):564-8.
The prevalence of serum markers of delta hepatitis was determined prospectively in 82 intravenous drug abusers at various stages of human immunodeficiency virus (HIV) disease. Seventeen were HIV negative, 30 were HIV positive without acquired immunodeficiency syndrome (AIDS) and 35 had been diagnosed as having AIDS. Antihepatitis D virus (HDV) in serum was measured by a commercially available enzyme-linked immunosorbent assay (ELISA) and also by solid phase capture radioimmunoassays (RIAs) for immunoglobulin G (IgG) and immunoglobulin M (IgM) anti-HDV. HDV antigen and hepatitis B virus (HBV) DNA were also measured. Hepatitis B surface antigen (HBsAg) and anti-HBs were determined by using a commercially available RIA. Anti-HDV (RIA) was only detected in serum that contained HBsAg. These anti-HDV (RIA) positive samples also tested positive with the commercial anti-HDV electroimmunoassay. In addition, the commercial anti-HDV ELISA detected anti-HDV in some serum samples that were negative for HBsAg; these anti HDV-positive HBsAg-negative samples were frequently lipemic or contained rheumatoid factor. The prevalence of HBsAg and anti-HBs did not differ significantly with the stage of HIV disease. HBsAg was detected in 3 of 13 (23%) HIV-negative, 5 of 29 (17%) HIV-positive, and 4 of 18 (22%) patients with AIDS. IgG and IgM anti-HDV (RIA) was positive in 2 of 3 HIV-negative and 4 of 5 HIV-positive pre-AIDS HBsAg-positive subjects. However, none of 4 AIDS patients had anti-HDV. The difference between AIDS and non-AIDS patients was statistically significant (Fisher's exact test, p = 0.03). HDV antigen was detected in serum from one AIDS patient.(ABSTRACT TRUNCATED AT 250 WORDS)
前瞻性地测定了82名处于人类免疫缺陷病毒（HIV）疾病不同阶段的静脉注射吸毒者中丁型肝炎血清标志物的流行情况。17人HIV阴性，30人HIV阳性但无获得性免疫缺陷综合征（AIDS），35人已被诊断患有AIDS。血清中的抗丁型肝炎病毒（HDV）通过市售酶联免疫吸附测定（ELISA）以及针对免疫球蛋白G（IgG）和免疫球蛋白M（IgM）抗HDV的固相捕获放射免疫测定（RIA）进行检测。还测定了HDV抗原和乙型肝炎病毒（HBV）DNA。使用市售RIA测定乙型肝炎表面抗原（HBsAg）和抗HBs。抗HDV（RIA）仅在含有HBsAg的血清中检测到。这些抗HDV（RIA）阳性样本在用市售抗HDV电免疫测定时也呈阳性。此外，市售抗HDV ELISA在一些HBsAg阴性的血清样本中检测到抗HDV；这些抗HDV阳性但HBsAg阴性的样本经常是脂血的或含有类风湿因子。HBsAg和抗HBs的流行率在HIV疾病阶段之间没有显著差异。在13名HIV阴性患者中有3名（23%）、29名HIV阳性患者中有5名（17%）以及18名AIDS患者中有4名（22%）检测到HBsAg。在3名HIV阴性且HBsAg阳性的AIDS前期患者中有2名以及5名HIV阳性且HBsAg阳性的AIDS前期患者中有4名，其IgG和IgM抗HDV（RIA）呈阳性。然而，4名AIDS患者中均无抗HDV。AIDS患者与非AIDS患者之间的差异具有统计学意义（Fisher精确检验，p = 0.03）。在一名AIDS患者的血清中检测到HDV抗原。（摘要截短于250字）
