Buragohain A K, Sung Y K, Coffin R S, Coutts R H
Biology Department, Imperial College of Science, Technology and Medicine, London, U.K.
J Gen Virol. 1994 Oct;75 ( Pt 10):2857-61. doi: 10.1099/0022-1317-75-10-2857.
Head-to-tail dimeric clones of both DNA A and DNA B of potato yellow mosaic geminivirus (PYMV) were constructed. These constructs were infectious when inoculated onto Nicotiana benthamiana plants either as DNA or by agroinoculation and were also infectious for tomato plants by agroinoculation. The dimers were not infectious for potato plants following inoculation by either method. Symptom induction required both DNA A and DNA B but agroinoculation with DNA A alone resulted in virus spread in 30% of the inoculated N. benthamiana plants. Leaf disc explants of N. benthamiana, tomato and potato could all be infected by agroinoculation indicating that the method of delivery of the DNA to intact potato plants was unsuitable for successful inoculation rather than an inherent inability of the virus to replicate/spread in potato per se. Neither whole plants nor leaf discs of sugar beet supported the replication of PYMV DNA.
构建了马铃薯黄花叶双生病毒（PYMV）的DNA A和DNA B的头对头二聚体克隆。这些构建体以DNA形式接种到本氏烟草植株上或通过农杆菌介导接种时具有感染性，并且通过农杆菌介导接种对番茄植株也具有感染性。通过这两种方法接种后，二聚体对马铃薯植株没有感染性。症状诱导需要DNA A和DNA B两者，但单独用DNA A进行农杆菌介导接种会导致30%的接种本氏烟草植株中病毒传播。本氏烟草、番茄和马铃薯的叶盘外植体都可以通过农杆菌介导接种而被感染，这表明将DNA递送至完整马铃薯植株的方法不适用于成功接种，而不是病毒本身在马铃薯中复制/传播的内在无能。甜菜的整株植物和叶盘都不支持PYMV DNA的复制。
