大鼠肠系膜动脉的组胺酶活性

Histaminase activity of mesenteric artery of the rat.

作者信息

Banchelli G, Ignesti G, Pirisino R, Raimondi L, Buffoni F

机构信息

Department of Pharmacology, University of Florence, Italy.

出版信息

J Neural Transm Suppl. 1994;41:445-8. doi: 10.1007/978-3-7091-9324-2_60.

DOI:10.1007/978-3-7091-9324-2_60

PMID:7931264

Abstract

In rat mesenteric artery homogenates histamine is oxidatively deaminated at high rate whereas putrescine is a poor substrate. The oxidation of histamine appears to be mainly catalyzed by an SSAO enzyme with high affinity for benzylamine (Bz.SSAO). Histamine oxidation is inhibited by B24 (2,5-diethoxy-4-aminomethylpyridine), a selective inhibitor of Bz.SSAO enzymes, and reduced by the presence of benzylamine. The Bz.SSAO enzyme which is present in the rat mesenteric artery is not only able to oxidize histamine, but also methylamine, acetylputrescine and some methylated forms of histamine including 1,4-methylhistamine.

摘要

在大鼠肠系膜动脉匀浆中，组胺以高速率被氧化脱氨，而腐胺则是一种较差的底物。组胺的氧化似乎主要由对苄胺具有高亲和力的SSAO酶（Bz.SSAO）催化。组胺氧化受到Bz.SSAO酶的选择性抑制剂B24（2,5 - 二乙氧基 - 4 - 氨基甲基吡啶）的抑制，并且由于苄胺的存在而减少。大鼠肠系膜动脉中存在的Bz.SSAO酶不仅能够氧化组胺，还能氧化甲胺、乙酰腐胺以及组胺的一些甲基化形式，包括1,4 - 甲基组胺。