Kobayashi S, Morgans C W, Casey J R, Kopito R R
Department of Biological Sciences, Stanford University, California 94305-5020.
J Neurosci. 1994 Oct;14(10):6266-79. doi: 10.1523/JNEUROSCI.14-10-06266.1994.
Plasma membrane anion exchangers constitute a multigene family that contributes to the regulation of intracellular pH and chloride concentration in many cell types. We have characterized two polypeptide isoforms of the AE3 gene that are expressed in the rat retina. Using antipeptide antibodies specific for defined NH2-terminal and COOH-terminal epitopes, we have identified a 165 kDa polypeptide whose expression is restricted to the primary glial cell type of the retina, the Müller cell, and a 125 kDa polypeptide that is expressed in horizontal neurons. Expression of the Müller cell isoform exhibits a polarized distribution and is highest in basal endfoot processes. These AE3 isoforms exhibit a distinct developmental expression pattern in postnatal rat retina. The neuronal isoform is undetectable in neonatal retina until postnatal day 10-15, correlating strongly with the onset of retinal function.
质膜阴离子交换蛋白构成一个多基因家族，在许多细胞类型中参与细胞内pH值和氯离子浓度的调节。我们已经鉴定出在大鼠视网膜中表达的AE3基因的两种多肽异构体。使用针对特定氨基末端和羧基末端表位的抗肽抗体，我们鉴定出一种165 kDa的多肽，其表达仅限于视网膜的主要神经胶质细胞类型，即穆勒细胞，以及一种在水平神经元中表达的125 kDa的多肽。穆勒细胞异构体的表达呈现极化分布，在基底终足突中最高。这些AE3异构体在出生后大鼠视网膜中表现出独特的发育表达模式。直到出生后第10 - 15天，新生视网膜中神经元异构体均无法检测到，这与视网膜功能的开始密切相关。
