Chopineau J, Sommier M F, Sautou V
Laboratoire de Pharmacie Clinique et Biotechnique, Faculté de Pharmacie, Clermont-Ferrand, France.
J Pharm Pharmacol. 1994 Jun;46(6):519-20. doi: 10.1111/j.2042-7158.1994.tb03842.x.
Use of a tourniquet on a limb to stem blood flow is frequent in surgery. However, this results in an ischaemia-reperfusion effect that causes damage through the production of free radicals. The aim of this work was to evaluate this production of free radicals in an ischaemia-reperfusion model in the rabbit after placement of a tourniquet. Plasma lipoperoxides were measured by high-performance liquid chromatography using the malondialdehyde assay method. We found significant (P < 0.01) production of free radicals 1 min after release of the tourniquet compared with controls; no variation in levels of free radicals was observed during the ischaemia phase.
在手术中，经常会使用止血带绑扎肢体以阻止血流。然而，这会导致缺血再灌注效应，通过自由基的产生造成损伤。这项研究的目的是评估在兔子身上使用止血带后缺血再灌注模型中自由基的产生情况。采用丙二醛检测法，通过高效液相色谱法测定血浆脂质过氧化物。我们发现，与对照组相比，松开止血带1分钟后自由基产生显著（P < 0.01）；在缺血阶段未观察到自由基水平的变化。
