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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Caulfield M P, Jones S, Vallis Y, Buckley N J, Kim G D, Milligan G, Brown D A

Wellcome Laboratory for Molecular Pharmacology, Department of Pharmacology, University College London.

J Physiol. 1994 Jun 15;477 ( Pt 3)(Pt 3):415-22. doi: 10.1113/jphysiol.1994.sp020203.

Microinjection of selective antibodies into superior cervical ganglion (SCG) neurones has identified the G-protein alpha-subunits mediating muscarinic receptor inhibition of M-type K+ current (IK(M)) and alpha-adrenoceptor inhibition of Ca2+ current (ICa). 2. Antibodies specific for G alpha q/11, but not those for G alpha o, reduced M-current inhibition by the muscarinic agonist oxotremorine-M, whereas anti-G alpha o antibodies, but not anti-G alpha q/11 or anti-G alpha i1-3 antibodies, reduced calcium current inhibition by noradrenaline. 3. Immunoblots with specific anti-G-protein antibodies demonstrated the presence of both G alpha q and G alpha 11, while G alpha o1 (but virtually no G alpha o2) was present. 4. We conclude that M1 muscarinic receptor inhibition of IK(M) is transduced by G alpha q and/or G alpha 11, and that G alpha o transduces alpha-adrenoceptor inhibition of ICa.

向上颈神经节（SCG）神经元微量注射选择性抗体，已鉴定出介导毒蕈碱受体对M型钾电流（IK(M)）抑制作用的G蛋白α亚基，以及介导α肾上腺素能受体对钙电流（ICa）抑制作用的G蛋白α亚基。2. 对Gαq/11特异的抗体可减弱毒蕈碱激动剂氧化震颤素-M对M电流的抑制作用，而对Gαo特异的抗体则不能；相反，对Gαo特异的抗体可减弱去甲肾上腺素对钙电流的抑制作用，而对Gαq/11或Gαi1-3特异的抗体则不能。3. 用特异的抗G蛋白抗体进行免疫印迹分析表明，同时存在Gαq和Gα11，且存在Gαo1（但几乎不存在Gαo2）。4. 我们得出结论，M1毒蕈碱受体对IK(M)的抑制作用是由Gαq和/或Gα11介导的，而Gαo介导α肾上腺素能受体对ICa的抑制作用。

Caulfield M P, Jones S, Vallis Y, Buckley N J, Kim G D, Milligan G, Brown D A

Wellcome Laboratory for Molecular Pharmacology, Department of Pharmacology, University College London.

J Physiol. 1994 Jun 15;477 ( Pt 3)(Pt 3):415-22. doi: 10.1113/jphysiol.1994.sp020203.

Microinjection of selective antibodies into superior cervical ganglion (SCG) neurones has identified the G-protein alpha-subunits mediating muscarinic receptor inhibition of M-type K+ current (IK(M)) and alpha-adrenoceptor inhibition of Ca2+ current (ICa). 2. Antibodies specific for G alpha q/11, but not those for G alpha o, reduced M-current inhibition by the muscarinic agonist oxotremorine-M, whereas anti-G alpha o antibodies, but not anti-G alpha q/11 or anti-G alpha i1-3 antibodies, reduced calcium current inhibition by noradrenaline. 3. Immunoblots with specific anti-G-protein antibodies demonstrated the presence of both G alpha q and G alpha 11, while G alpha o1 (but virtually no G alpha o2) was present. 4. We conclude that M1 muscarinic receptor inhibition of IK(M) is transduced by G alpha q and/or G alpha 11, and that G alpha o transduces alpha-adrenoceptor inhibition of ICa.

向上颈神经节（SCG）神经元微量注射选择性抗体，已鉴定出介导毒蕈碱受体对M型钾电流（IK(M)）抑制作用的G蛋白α亚基，以及介导α肾上腺素能受体对钙电流（ICa）抑制作用的G蛋白α亚基。2. 对Gαq/11特异的抗体可减弱毒蕈碱激动剂氧化震颤素-M对M电流的抑制作用，而对Gαo特异的抗体则不能；相反，对Gαo特异的抗体可减弱去甲肾上腺素对钙电流的抑制作用，而对Gαq/11或Gαi1-3特异的抗体则不能。3. 用特异的抗G蛋白抗体进行免疫印迹分析表明，同时存在Gαq和Gα11，且存在Gαo1（但几乎不存在Gαo2）。4. 我们得出结论，M1毒蕈碱受体对IK(M)的抑制作用是由Gαq和/或Gα11介导的，而Gαo介导α肾上腺素能受体对ICa的抑制作用。