Yamada M, Shimizu K, Miyao Y, Hayakawa T, Nakajima K, Nakahira K, Nakagawa H, Mikoshiba K, Ikenaka K
Department of Neurosurgery, Center for Adult Diseases, Osaka, Japan.
J Neurosci Res. 1994 Jul 1;38(4):415-23. doi: 10.1002/jnr.490380407.
Murine RSV-M glioma cells were genetically labeled with a retroviral BAG vector carrying the Escherichia coli beta-galactosidase gene. The X-gal-positive stable cell line RSV-M/BAG was obtained by the FDG-FACS method. To examine the behavior of glioma cells in the brain, we homografted RSV-M/BAG cells into the brain of C3H/HeN mice as cell suspensions. Individual grafted glioma cells were easily detected by histochemical staining for B-galactosidase (beta-gal). Three days after grafting, the beta-gal-positive cells were mainly found in the subependymal zone of the lateral ventricle. In addition, some solitary labeled cells were found at locations distant from the injection sites. On the seventh day after implantation, tumor masses were observed and graft-derived glioma cells were migrating bilaterally along the fibers in the corpus callosum. Other labeled cells extended into the brain parenchyma via the perivascular (Virchow-Robin) spaces. Rapid and extensive migration of individual glioma cells was thus clearly demonstrated by intracerebral transplantation of RSV-M/BAG cells.
用携带大肠杆菌β-半乳糖苷酶基因的逆转录病毒BAG载体对鼠源RSV-M胶质瘤细胞进行基因标记。通过荧光激活细胞分选（FDG-FACS）方法获得了X-gal阳性稳定细胞系RSV-M/BAG。为了研究胶质瘤细胞在脑内的行为，我们将RSV-M/BAG细胞作为细胞悬液移植到C3H/HeN小鼠脑内。通过β-半乳糖苷酶（β-gal）的组织化学染色很容易检测到单个移植的胶质瘤细胞。移植后3天，β-gal阳性细胞主要位于侧脑室室管膜下区。此外，在远离注射部位的地方发现了一些单个的标记细胞。植入后第7天，观察到肿瘤块，移植来源的胶质瘤细胞沿胼胝体纤维向双侧迁移。其他标记细胞通过血管周围（Virchow-Robin）间隙延伸至脑实质。因此，通过RSV-M/BAG细胞的脑内移植清楚地证明了单个胶质瘤细胞的快速广泛迁移。
