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非洲爪蟾p53的功能特性：温度敏感型反式激活而非抑制的证据。

Functional characterization of Xenopus laevis p53: evidence of temperature-sensitive transactivation but not of repression.

作者信息

Ridgway P J, Soussi T, Braithwaite A W

机构信息

Division of Cell Biology, John Curtin School of Medical Research, Australian National University, Canberra.

出版信息

J Virol. 1994 Nov;68(11):7178-87. doi: 10.1128/JVI.68.11.7178-7187.1994.

DOI:10.1128/JVI.68.11.7178-7187.1994
PMID:7933100
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC237157/
Abstract

We have investigated the effect of Xenopus laevis p53 (Xp53) on transcription from a variety of promoters which are regulated by mouse p53 using a chloramphenicol acetyltransferase reporter system. Although Xp53 transactivated promoters that are up-regulated by mouse p53, it was unable to cause repression. This ability to transactivate gene expression was dependent on a temperature of 32 degrees C, and activity was lost at 37 degrees C. Temperature-sensitive transactivation was correlated with temperature-dependent binding of Xp53 to the adenovirus E1B58K protein. Despite the marked loss of transcriptional activation and binding to E1B58K at 37 degrees C, Xp53 was still capable of binding simian virus 40 large T antigen and inhibiting simian virus 40 origin-dependent DNA replication. These data show that Xp53 is temperature sensitive for N-terminal activities and suggest that the transactivation and repression "domains" of p53 are distinct.

摘要

我们利用氯霉素乙酰转移酶报告系统，研究了非洲爪蟾p53（Xp53）对多种受小鼠p53调控的启动子转录的影响。尽管Xp53能反式激活那些被小鼠p53上调的启动子，但它无法引起抑制作用。这种反式激活基因表达的能力依赖于32摄氏度的温度，在37摄氏度时活性丧失。温度敏感性反式激活与Xp53在温度依赖下与腺病毒E1B58K蛋白的结合相关。尽管在37摄氏度时转录激活和与E1B58K的结合显著丧失，但Xp53仍能够结合猿猴病毒40大T抗原并抑制猿猴病毒40起源依赖性DNA复制。这些数据表明Xp53对N端活性具有温度敏感性，并提示p53的反式激活和抑制“结构域”是不同的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14b7/237157/4f255b83b3cf/jvirol00020-0373-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14b7/237157/c75294538920/jvirol00020-0370-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14b7/237157/68369ad29a24/jvirol00020-0370-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14b7/237157/1886f7f58bb4/jvirol00020-0371-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14b7/237157/4f255b83b3cf/jvirol00020-0373-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14b7/237157/c75294538920/jvirol00020-0370-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14b7/237157/68369ad29a24/jvirol00020-0370-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14b7/237157/1886f7f58bb4/jvirol00020-0371-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14b7/237157/4f255b83b3cf/jvirol00020-0373-a.jpg

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本文引用的文献

1
The p53 activation domain binds the TATA box-binding polypeptide in Holo-TFIID, and a neighboring p53 domain inhibits transcription.p53激活结构域与全酶TFIID中的TATA盒结合多肽结合，且相邻的p53结构域抑制转录。
Mol Cell Biol. 1993 Jun;13(6):3291-300. doi: 10.1128/mcb.13.6.3291-3300.1993.
2
Wild-type but not mutant p53 can repress transcription initiation in vitro by interfering with the binding of basal transcription factors to the TATA motif.野生型而非突变型p53可通过干扰基础转录因子与TATA基序的结合在体外抑制转录起始。
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癌蛋白MDM2掩盖了肿瘤抑制因子p53的激活结构域。
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Cancer. A death in the life of p53.癌症。p53生命中的死亡。
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p53 confers a selective advantage on transfected HeLa cells.p53赋予转染的HeLa细胞选择性优势。
Oncogene. 1993 Apr;8(4):1069-74.
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Physical and functional interaction between WT1 and p53 proteins.WT1蛋白与p53蛋白之间的物理和功能相互作用。
Proc Natl Acad Sci U S A. 1993 Jun 1;90(11):5100-4. doi: 10.1073/pnas.90.11.5100.
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Wild-type mouse p53 down-regulates transcription from different virus enhancer/promoters.野生型小鼠p53可下调来自不同病毒增强子/启动子的转录。
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Inhibition of DNA replication factor RPA by p53.p53对DNA复制因子RPA的抑制作用。
Nature. 1993 Sep 2;365(6441):79-82. doi: 10.1038/365079a0.
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Tumour suppressor genes. No room at the p53 inn.肿瘤抑制基因。p53 之“店”已无空位。
Nature. 1993 Sep 2;365(6441):17-8. doi: 10.1038/365017a0.
10
A comparison of the biological activities of wild-type and mutant p53.野生型和突变型p53的生物活性比较。
FASEB J. 1993 Jul;7(10):855-65. doi: 10.1096/fasebj.7.10.8344485.